干旱区生态环境知识资源中心(Arid): Relationship between microbial diversity and nitrogenase activity of Stipagrostis pennata rhizosheath

Arid

DOI	10.1002/jcb.28625
	Relationship between microbial diversity and nitrogenase activity of Stipagrostis pennata rhizosheath
	Tian, Yongzhi 1; Ma, Xiaolin 1; Li, Yuanting 1; Cheng, Cong 2; Ge, Fengwei 1; An, Dengdi 1
通讯作者	An, Dengdi
来源期刊	JOURNAL OF CELLULAR BIOCHEMISTRY
ISSN	0730-2312
EISSN	1097-4644
出版年	2019
卷号	120 期号:8 页码:13501-13508
英文摘要	Nitrogen is the key factor for plant survival and growth, especially in the desert. Stipagrostis pennata, a sand born drought-resistant plant, could colonize pioneerly in Gurbantunggut Desert during revegetation. One strategy for their environment adaptation was the rhizosheath formatted by root-hair, mucilaginous exudates, microbial components, and soil particles, for which not only provides a favorable living microenvironment but also supplies essential nutrients. To understand the relationship between microorganisms living in rhizosheaths and the nitrogen nutrition supply, the microbial diversity and nitrogenase activity was estimated during the growth of S. pennata. Five samples of the rhizosheath, which based on the development periods of the plant, regreen, flowering, filling, seed maturating, and withering period, were collected. The nitrogenase activity was estimated by acetylene reduction and the microbial diversity was analyzed by 16S rRNA high-throughput sequencing. The results showed that the nitrogenase activity was increased slowly during regreen to flowering, while reached a peak rapidly at filling sample and then decreased gradually. A total of 274 operational taxonomic units (OTUs) were identified and significant differences in community structure and composition at each growth period. Among them, the main phyla included Actinobacteria and Proteus, which were the most abundant phyla in all periods. In addition, the microbial diversity in the grain filling period was higher than other periods in view of the analysis of alpha diversity and beta diversity. Furthermore, principal component analysis (PCA) analysis showed that the microbial communities in the filling period was low in similarity with other periods. Most importantly, the OTUs associated with nitrogen fixation is the most during the filling period, involving Phagecidae and Fucoraceae. Overall, the study not only revealed the differences in nitrogenase activity among different developmental periods in S. pennata, but also explored the potential bridges between it and community structure and diversity of bacteria.
英文关键词	16S rRNA Miseq microbiome nitrogenase activity Stipagrostis pennata
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000471718300138
WOS关键词	COMMUNITY STRUCTURE ; REMOVAL ; LEGUME ; PLANTS
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216831
作者单位	1.Xinjiang Normal Univ, Coll Life Sci, Xinjiang Key Lab Special Species Conservat & Regu, Urumqi 830054, Peoples R China; 2.Nanjing Agr Univ, Coll Life Sci, Jiangsu Key Lab Microbiol & Funct Genom, Nanjing, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Tian, Yongzhi,Ma, Xiaolin,Li, Yuanting,et al. Relationship between microbial diversity and nitrogenase activity of Stipagrostis pennata rhizosheath[J],2019,120(8):13501-13508.
APA	Tian, Yongzhi,Ma, Xiaolin,Li, Yuanting,Cheng, Cong,Ge, Fengwei,&An, Dengdi.(2019).Relationship between microbial diversity and nitrogenase activity of Stipagrostis pennata rhizosheath.JOURNAL OF CELLULAR BIOCHEMISTRY,120(8),13501-13508.
MLA	Tian, Yongzhi,et al."Relationship between microbial diversity and nitrogenase activity of Stipagrostis pennata rhizosheath".JOURNAL OF CELLULAR BIOCHEMISTRY 120.8(2019):13501-13508.