干旱区生态环境知识资源中心(Arid): Real-time quantitative detection of Vampirovibrio chlorellavorus, an obligate bacterial pathogen of Chlorella sorokiniana

Arid

DOI	10.1007/s10811-018-1659-z
	Real-time quantitative detection of Vampirovibrio chlorellavorus, an obligate bacterial pathogen of Chlorella sorokiniana
	Steichen, Seth A.; Brown, Judith K.
通讯作者	Brown, Judith K.
来源期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
EISSN	1573-5176
出版年	2019
卷号	31 期号:2 页码:1117-1129
英文摘要	Vampirovibrio chlorellavorus is an obligate, predatory bacterial pathogen of the genus Chlorella. It is recognized as an important pathogen of Chlorella sorokiniana, field isolate DOE 1412, a highly-favored microalga for cultivation in outdoor reactors in the arid USA Southwest for feedstocks used in biofuel production. To determine the V. chlorellavorus titer, based on gene copy number, required to cause infection and mortality of C. sorokiniana in an experimental outdoor reactor, a multiplexed quantitative polymerase chain reaction (qPCR) assay was developed for pathogen detection, based on the 16S and 18S ribosomal RNA gene of V. chlorellavorus and C. sorokiniana, respectively. The assay was further used to establish the optimal effective concentration of benzalkonium chloride required to achieve a below disease-threshold-bacterial titer, while minimizing biocidal effects on algal growth and enable economic biomass production. Reactors treated with 2.0 ppm benzalkonium chloride at four-day intervals throughout the cultivation cycle experienced runs of 22 days or longer, compared to 12 days for the untreated control. The qPCR assay was used to estimate disease severity over time using the Area Under the Disease Progress Stairs (AUDPS) metric, indicating a severity rating of 0.016 and 62.308 in biocide-treated and untreated cultures, respectively. The near-real time assay detected as few as 13 copies of V. chlorellavorus, allowing for the recognition of its presence in the reactor just before algal cell density decreased, an indication of pathogen attack, while also informing the timing of biocide applications to minimize DOE 1412 infection such that harvestable biomass could be produced.
英文关键词	Biocide Melainabacteria Microalgal cultivation Polymerase chain reaction Vampirovibrionales
类型	Article
语种	英语
国家	USA
开放获取类型	Green Published
收录类别	SCI-E
WOS记录号	WOS:000463988500030
WOS关键词	BDELLOVIBRIO-CHLORELLAVORUS ; BENZALKONIUM CHLORIDE ; GROWTH ; MODEL ; PCR ; QUANTIFICATION ; IDENTIFICATION ; AMPLIFICATION ; BIOFUELS ; PRIMERS
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
来源机构	University of Arizona
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216689
作者单位	Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA
推荐引用方式 GB/T 7714	Steichen, Seth A.,Brown, Judith K.. Real-time quantitative detection of Vampirovibrio chlorellavorus, an obligate bacterial pathogen of Chlorella sorokiniana[J]. University of Arizona,2019,31(2):1117-1129.
APA	Steichen, Seth A.,&Brown, Judith K..(2019).Real-time quantitative detection of Vampirovibrio chlorellavorus, an obligate bacterial pathogen of Chlorella sorokiniana.JOURNAL OF APPLIED PHYCOLOGY,31(2),1117-1129.
MLA	Steichen, Seth A.,et al."Real-time quantitative detection of Vampirovibrio chlorellavorus, an obligate bacterial pathogen of Chlorella sorokiniana".JOURNAL OF APPLIED PHYCOLOGY 31.2(2019):1117-1129.