干旱区生态环境知识资源中心(Arid): Isolation of depsipeptides and optimization for enhanced production of valinomycin from the North-Western Himalayan cold desert strain Streptomyces lavendulae

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DOI	10.1038/s41429-019-0183-y
	Isolation of depsipeptides and optimization for enhanced production of valinomycin from the North-Western Himalayan cold desert strain Streptomyces lavendulae
	Singh, Varun P.1,2; Sharma, Richa 3,4; Sharma, Vandana 4,5; Raina, Chand 3; Kapoor, Kamal K.6; Kumar, Anil 2; Chaubey, Asha 3,4; Singh, Deepika 1,4,5; Vishwakarma, Ram A.1,4
通讯作者	Chaubey, Asha ; Singh, Deepika ; Vishwakarma, Ram A.
来源期刊	JOURNAL OF ANTIBIOTICS
ISSN	0021-8820
EISSN	1881-1469
出版年	2019
卷号	72 期号:8 页码:617-624
英文摘要	Exploration of microbial dynamics of Streptomyces lavendulae ACR-DA1, a psychrotrophic isolate from the North-Western Himalayan cold desert, was carried out using matrix-assisted laser desorbtion ionisation-time of flight mass spectrometer. Valinomycin was found as a major produce and cyclic depsipeptide montanastatin as a minor produce. The yield of the valinomycin was found to be 0.3 mg l(-1) in submerged growth condition at the batch scale. Miniaturization of optimization experiments was adept to maximize the production using the expeditious and efficient technique of intact cell mass spectrometry. The present study showed that using optimized conditions and growing the culture in synthetic mineral base starch medium at 10 degrees C enhanced the production to 19.4 mg l(-1). Our results demonstrated 64-fold increase in yield from the wild-type S. lavendulae ACR-DA1 strain using a simple and economical downstream process.
类型	Article
语种	英语
国家	India
收录类别	SCI-E
WOS记录号	WOS:000477713000005
WOS类目	Biotechnology & Applied Microbiology ; Immunology ; Microbiology ; Pharmacology & Pharmacy
WOS研究方向	Biotechnology & Applied Microbiology ; Immunology ; Microbiology ; Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216670
作者单位	1.Indian Inst Integrat Med, Med Chem Div, CSIR, Canal Rd, Jammu 180001, Jammu & Kashmir, India; 2.Shri Mata Vaishno Devi Univ, Dept Biotechnol, Fac Sci, Katra 182320, Jammu & Kashmir, India; 3.Indian Inst Integrat Med, CSIR, Fermentat Technol Div, Canal Rd, Jammu 180001, Jammu & Kashmir, India; 4.Acad Sci & Innovat Res, Jammu 180001, Jammu & Kashmir, India; 5.Indian Inst Integrat Med, CSIR, Qual Control & Qual Assurance, Canal Rd, Jammu 180001, Jammu & Kashmir, India; 6.Univ Jammu, Dept Chem, Jammu 180006, Jammu & Kashmir, India
推荐引用方式 GB/T 7714	Singh, Varun P.,Sharma, Richa,Sharma, Vandana,et al. Isolation of depsipeptides and optimization for enhanced production of valinomycin from the North-Western Himalayan cold desert strain Streptomyces lavendulae[J],2019,72(8):617-624.
APA	Singh, Varun P..,Sharma, Richa.,Sharma, Vandana.,Raina, Chand.,Kapoor, Kamal K..,...&Vishwakarma, Ram A..(2019).Isolation of depsipeptides and optimization for enhanced production of valinomycin from the North-Western Himalayan cold desert strain Streptomyces lavendulae.JOURNAL OF ANTIBIOTICS,72(8),617-624.
MLA	Singh, Varun P.,et al."Isolation of depsipeptides and optimization for enhanced production of valinomycin from the North-Western Himalayan cold desert strain Streptomyces lavendulae".JOURNAL OF ANTIBIOTICS 72.8(2019):617-624.