干旱区生态环境知识资源中心(Arid): Lathyrus sativus Originating from Different Geographical Regions Reveals Striking Differences in Kunitz and Bowman-Birk Inhibitor Activities

Arid

DOI	10.1021/acs.jafc.9b02604
	Lathyrus sativus Originating from Different Geographical Regions Reveals Striking Differences in Kunitz and Bowman-Birk Inhibitor Activities
	Xu, Quanle 1,2; Qu, Jinmiao 1; Song, Bo 2,3; Liu, Fengjuan 1; Chen, Peng 1; Krishnan, Hari B.2
通讯作者	Krishnan, Hari B.
来源期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	0021-8561
EISSN	1520-5118
出版年	2019
卷号	67 期号:29 页码:8119-8129
英文摘要	Grass pea (Lathyrus sativus L.) is an important legume commonly grown in arid and semi-arid regions. This protein-rich legume performs well even under harsh environmental conditions and is considered to be a strategic famine food in developing countries. Unfortunately, its potential usage is greatly limited as a result of the presence of antinutritional factors, including the neuroexcitatory amino acid beta-N-oxalyl-L-alpha,beta-diaminopropionic acid (beta-ODAP) and protease inhibitors. beta-ODAP is responsible for a neurodegenerative syndrome that results in the paralysis of lower limbs, while protease inhibitors affect protein digestibility, resulting in reduced growth. Concerted research efforts have led to development of grass pea cultivars with reduced beta-ODAP content. In contrast, very little information is available on the protease inhibitors of L. sativus. In this study, we have conducted biochemical characterization of 51 L. sativus accessions originating from different geographical regions. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analyses of seed globulins and prolamins revealed striking similarity in their protein profile, although geographic-specific variations in profiles was also evident. Measurement of Bowman-Birk chymotrypsin inhibitor (BBi) and Kunitz trypsin inhibitor (KTi) activities in accessions revealed striking differences among them. Amino acid sequence alignment of grass pea BBi and KTi revealed significant homology to protease inhibitors from several legumes. Real-time polymerase chain reaction analysis demonstrated high-level expression of BBi and KTi in dry seeds and weak expression in other organs. Our study demonstrates substantial variation in BBi and KTi among grass pea accessions that could be exploited in breeding programs for the development of grass pea lines that are devoid of these antinutritional factors.
英文关键词	grass pea storage protein antinutritional factors beta-N-oxalyl-L-alpha,beta-diaminopropionic acid Kunitz trypsin inhibitor Bowman-Birk inhibitor
类型	Article
语种	英语
国家	Peoples R China ; USA
收录类别	SCI-E
WOS记录号	WOS:000477788100006
WOS关键词	TRYPSIN-INHIBITOR ; GRASS-PEA ; PROTEASE INHIBITORS ; BETA-ODAP ; PROTEINASE-INHIBITOR ; DISULFIDE BRIDGE ; STORAGE PROTEINS ; CICERA L. ; SEED ; PURIFICATION
WOS类目	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216639
作者单位	1.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China; 2.Univ Missouri, Plant Genet Res, USDA, ARS, Columbia, MO 65211 USA; 3.Northeast Agr Univ, Chinese Minist Educ, Key Lab Soybean Biol, Harbin 150030, Heilongjiang, Peoples R China
推荐引用方式 GB/T 7714	Xu, Quanle,Qu, Jinmiao,Song, Bo,et al. Lathyrus sativus Originating from Different Geographical Regions Reveals Striking Differences in Kunitz and Bowman-Birk Inhibitor Activities[J]. 西北农林科技大学,2019,67(29):8119-8129.
APA	Xu, Quanle,Qu, Jinmiao,Song, Bo,Liu, Fengjuan,Chen, Peng,&Krishnan, Hari B..(2019).Lathyrus sativus Originating from Different Geographical Regions Reveals Striking Differences in Kunitz and Bowman-Birk Inhibitor Activities.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(29),8119-8129.
MLA	Xu, Quanle,et al."Lathyrus sativus Originating from Different Geographical Regions Reveals Striking Differences in Kunitz and Bowman-Birk Inhibitor Activities".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.29(2019):8119-8129.