干旱区生态环境知识资源中心(Arid): Physiological validation of a non-invasive method to evaluate adrenocortical activity and the time course for the excretion of stress hormones in the feces of three species of desert gerbils

Arid

DOI	10.1163/22244662-20191043
	Physiological validation of a non-invasive method to evaluate adrenocortical activity and the time course for the excretion of stress hormones in the feces of three species of desert gerbils
	St Juliana, Justin R.1,2; Bryant, Jocelyn L.3; Wielebnowski, Nadja 3,4; Kotler, Burt P.2
通讯作者	St Juliana, Justin R.
来源期刊	ISRAEL JOURNAL OF ECOLOGY & EVOLUTION
ISSN	1565-9801
EISSN	2224-4662
出版年	2019
卷号	65 期号:1-2 页码:21-27
英文摘要	We evaluated the suitability of a corticosterone enzyme immunoassay (EIA) to monitor excretion of fecal glucocorticoid metabolites (FGM) in response to Adrenocorticotropic hormone (ACTH) and saline injections in three desert rodent species (Gerbillus andersoni allenbyi (GA), Gerbillus nanus (GN), and Gerbilis piridium (GP). We exposed 24 gerbils (N = 9 for GA, N = 7 for GN, N = 8 for GP) to an ACTH and a saline injection at different times. Fecal samples were collected hourly for 24 hours after injection. The average starting concentration (baseline) FGM concentration was 797 ng/g for GA, 183 ng/g for GN, and 749 ng/g for GP. The average peak concentration was 2377 ng/g for GA, 589 ng/g for GN, and 1987 ng/g for GP. We were able to provide a physiological validation for the chosen assay in GAs and GPs, however, our results for GNs were less clear. We found an increase in FGM concentrations on average after 5.5 hours in GA, 3.1 hours in GN, and 3.8 hours in GP. We found a peak in FGM concentration on average after 8.8 hours in GA, 5.6 hours in GN, and 10.3 hours in GP. We determined that FGM concentration returned to starting value on average after 14.4 hours in GA, 9.1 hours in GN, and 15.1 hours in GP. The outcomes of this study can help establish trapping protocols and time frames for FGM monitoring of these wild small mammal populations. The time course for excretion of FGM is similar between the species in this study, and comparable to some non-desert rodents. We found high variation in the time course of excretion within species. This variation needs to be taken into account when monitoring stress responses in the field. By assessing adrenocortical activity using FGM monitoring, stress responses to varying ecological and environmental factors can be reliably examined in the field.
英文关键词	desert feces glucocorticoids rodent stress hormones
类型	Article
语种	英语
国家	USA ; Israel
收录类别	SCI-E
WOS记录号	WOS:000463917900003
WOS关键词	FECAL GLUCOCORTICOID METABOLITES ; HABITAT SELECTION ; CORTICOSTERONE ; MAMMALS ; MICE ; SEX ; CONSERVATION ; EXTRACTION ; BEHAVIOR ; STEROIDS
WOS类目	Ecology ; Evolutionary Biology
WOS研究方向	Environmental Sciences & Ecology ; Evolutionary Biology
来源机构	Ben-Gurion University of the Negev
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216575
作者单位	1.Indiana State Univ, Dept Biol, 200 North Seventh St, Terre Haute, IN 47809 USA; 2.Ben Gurion Univ Negev, Mitrani Dept Desert Ecol, Jacob Blaustein Inst Desert Res, IL-84990 Midreshet Ben Gurion, Israel; 3.Chicago Zool Soc, Brookfield Zoo, Conservat Sci Dept, 3300 Golf Rd, Brookfield, IL 60513 USA; 4.Oregon Zoo, Dept Conservat & Res, 4001 SW Canyon Rd, Portland, OR 97221 USA
推荐引用方式 GB/T 7714	St Juliana, Justin R.,Bryant, Jocelyn L.,Wielebnowski, Nadja,et al. Physiological validation of a non-invasive method to evaluate adrenocortical activity and the time course for the excretion of stress hormones in the feces of three species of desert gerbils[J]. Ben-Gurion University of the Negev,2019,65(1-2):21-27.
APA	St Juliana, Justin R.,Bryant, Jocelyn L.,Wielebnowski, Nadja,&Kotler, Burt P..(2019).Physiological validation of a non-invasive method to evaluate adrenocortical activity and the time course for the excretion of stress hormones in the feces of three species of desert gerbils.ISRAEL JOURNAL OF ECOLOGY & EVOLUTION,65(1-2),21-27.
MLA	St Juliana, Justin R.,et al."Physiological validation of a non-invasive method to evaluate adrenocortical activity and the time course for the excretion of stress hormones in the feces of three species of desert gerbils".ISRAEL JOURNAL OF ECOLOGY & EVOLUTION 65.1-2(2019):21-27.