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	Molecular Detection of Leishmania spp. in Skin and Blood of Stray Dogs from Endemic Areas of Cutaneous Leishmaniasis in Saudi Arabia
	Alanazi, Abdullah D.1; Puschendorf, Robert 2; Alyousif, Mohamed S.3; Al-Khalifa, Mohamed S.3; Alharbi, Samir A.1; AL-Shehri, Zafer S.1; Said, Ashraf E.1; Alanazi, Ibrahim O.4; AL-Mohammed, Hamdan, I 5; Alraey, Yasser A.6
通讯作者	Alanazi, Abdullah D.
来源期刊	IRANIAN JOURNAL OF PARASITOLOGY
ISSN	1735-7020
EISSN	2008-238X
出版年	2019
卷号	14 期号:2 页码:231-239
英文摘要	Background: Dogs can act as reservoirs of canine leishmaniasis, caused by Leishmania species. The aims of this study were to determine the prevalence of canine leishmaniasis using a PCR technique among stray dogs living in three provinces of Saudi Arabia, Riyadh, Al-Ahsa Oasis and Al-Qaseem, where the disease is endemic; and to identify and document different Leishmania to species levels Methods: This cross-sectional investigation was conducted, from Mar 2016 to Apr 2018, in three parts of Saudi Arabia: Central province (Riyadh), Eastern province (Al-Ahsa Oasis) and Al-Qaseem province. Blood samples were collected from 526 dogs; 40 presented cutaneous nodules so were suspected clinically of cutaneous leishmaniasis. Biopsy tissue collections and parasite cultures were performed. A generic kDNA was performed using different primers for Leishmania differentiation. Results: All blood samples were negative for Leishmania infantum infection by molecular analysis, though forty dogs had thick cutaneous lesions in different parts of their body. Four dogs' skin lesions were associated with dermatitis, splenomegaly and lymphadenomegaly. Parasite culture was used to diagnose cutaneous leishmaniasis, identifying 31/40 (77.5%) positive samples. Overall, of 526 samples, the prevalence of L. major and L. tropica was found to be 4% and 1.9%, respectively. Gender and age had a significant effect on Leishmania prevalence: (P=0.0212 and 0.0357), respectively. Conclusion: This was the first molecular study of dog leishmaniasis from Saudi Arabia of dogs confirmed to have cutaneous leishmaniasis. Further epidemiological and molecular investigations of domestic and wild canine infections with L. major, L. tropica and L. infantum in endemic and nonendemic areas of Saudi Arabia are required, for leishmaniasis control.
英文关键词	Leishmania Dogs kDNA Saudi Arabia
类型	Article
语种	英语
国家	Saudi Arabia ; England
开放获取类型	gold, Green Submitted, Green Accepted
收录类别	SCI-E
WOS记录号	WOS:000471918300006
WOS关键词	CANINE VISCERAL LEISHMANIASIS ; INFECTING MAN ; WILD ANIMALS ; AL-HASSA ; PCR ; IDENTIFICATION ; DIAGNOSIS ; INFANTUM ; TROPICA ; IRAN
WOS类目	Parasitology
WOS研究方向	Parasitology
来源机构	King Saud University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216550
作者单位	1.Shaqra Univ, Dept Biol Sci, Fac Sci & Humanities, Ad Dawadimi, Saudi Arabia; 2.Plymouth Univ, Sch Biol Sci, Plymouth, Devon, England; 3.King Saud Univ, Coll Sci, Dept Zool, Riyadh, Saudi Arabia; 4.King Abdulaziz City Sci & Technol, Natl Ctr Genom Technol, Riyadh, Saudi Arabia; 5.King Faisal Univ, Fac Med, Dept Parasitol, Al Hasa, Saudi Arabia; 6.Liverpool Sch Trop Medidne, Dept Parasitol, Liverpool, Merseyside, England
推荐引用方式 GB/T 7714	Alanazi, Abdullah D.,Puschendorf, Robert,Alyousif, Mohamed S.,et al. Molecular Detection of Leishmania spp. in Skin and Blood of Stray Dogs from Endemic Areas of Cutaneous Leishmaniasis in Saudi Arabia[J]. King Saud University,2019,14(2):231-239.
APA	Alanazi, Abdullah D..,Puschendorf, Robert.,Alyousif, Mohamed S..,Al-Khalifa, Mohamed S..,Alharbi, Samir A..,...&Alraey, Yasser A..(2019).Molecular Detection of Leishmania spp. in Skin and Blood of Stray Dogs from Endemic Areas of Cutaneous Leishmaniasis in Saudi Arabia.IRANIAN JOURNAL OF PARASITOLOGY,14(2),231-239.
MLA	Alanazi, Abdullah D.,et al."Molecular Detection of Leishmania spp. in Skin and Blood of Stray Dogs from Endemic Areas of Cutaneous Leishmaniasis in Saudi Arabia".IRANIAN JOURNAL OF PARASITOLOGY 14.2(2019):231-239.