干旱区生态环境知识资源中心(Arid): Enhancement of heavy metal tolerance and accumulation efficiency by expressing Arabidopsis ATP sulfurylase gene in alfalfa

Arid

DOI	10.1080/15226514.2019.1606784
	Enhancement of heavy metal tolerance and accumulation efficiency by expressing Arabidopsis ATP sulfurylase gene in alfalfa
	Kumar, V; AlMomin, S.; Al-Shatti, A.; Al-Aqeel, H.; Al-Salameen, F.; Shajan, A. B.; Nair, S. M.
通讯作者	Kumar, V
来源期刊	INTERNATIONAL JOURNAL OF PHYTOREMEDIATION
ISSN	1522-6514
EISSN	1549-7879
出版年	2019
卷号	21 期号:11 页码:1112-1121
英文摘要	Transgenic alfalfa (Medicago sativa L.) plants overexpressing the Arabidopsis ATP sulfurylase gene were generated using Agrobacterium-mediated genetic transformation to enhance their heavy metal accumulation efficiency. The ATP sulfurylase gene was cloned from Arabidopsis, following exposure to vanadium (V) and lead (Pb), and transferred into an Agrobacterium tumefaciens binary vector. This was co-cultivated with leaf explants of the alfalfa genotype Regen SY. Co-cultivated leaf explants were cultured on callus and somatic embryo induction medium, followed by regeneration medium for regenerating complete transgenic plants. The transgenic nature of the plants was confirmed using PCR and southern hybridization. The expression of Arabidopsis ATP sulfurylase gene in the transgenic plants was evaluated through RT-PCR. The selected transgenic lines showed increased tolerance to a mixture of five heavy metals and also demonstrated enhanced metal uptake ability under controlled conditions. The transgenic lines were fertile and did not exhibit any apparent morphological abnormality. The results of this study indicated an effective approach to improve the heavy metal accumulation ability of alfalfa plants which can then be used for the remediation of contaminated soil in arid regions.
英文关键词	Arabidopsis ATP sulfurylase genetic engineering phytoremediation
类型	Article
语种	英语
国家	Kuwait
收录类别	SCI-E
WOS记录号	WOS:000469653900001
WOS关键词	AGROBACTERIUM-MEDIATED TRANSFORMATION ; OIL-CONTAMINATED SOIL ; INDIAN MUSTARD PLANTS ; SOMATIC EMBRYOGENESIS ; TRANSGENIC PLANTS ; MESOPHYLL PROTOPLASTS ; SULFATE TRANSPORTER ; DEGRADED SOILS ; PHYTOREMEDIATION ; REMEDIATION
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216486
作者单位	Kuwait Inst Sci Res, Environm & Life Sci Res Ctr, Biotechnol Program, Kuwait 13109, Kuwait
推荐引用方式 GB/T 7714	Kumar, V,AlMomin, S.,Al-Shatti, A.,et al. Enhancement of heavy metal tolerance and accumulation efficiency by expressing Arabidopsis ATP sulfurylase gene in alfalfa[J],2019,21(11):1112-1121.
APA	Kumar, V.,AlMomin, S..,Al-Shatti, A..,Al-Aqeel, H..,Al-Salameen, F..,...&Nair, S. M..(2019).Enhancement of heavy metal tolerance and accumulation efficiency by expressing Arabidopsis ATP sulfurylase gene in alfalfa.INTERNATIONAL JOURNAL OF PHYTOREMEDIATION,21(11),1112-1121.
MLA	Kumar, V,et al."Enhancement of heavy metal tolerance and accumulation efficiency by expressing Arabidopsis ATP sulfurylase gene in alfalfa".INTERNATIONAL JOURNAL OF PHYTOREMEDIATION 21.11(2019):1112-1121.