干旱区生态环境知识资源中心(Arid): Genome Editing in Cowpea Vigna unguiculata Using CRISPR-Cas9

Arid

DOI	10.3390/ijms20102471
	Genome Editing in Cowpea Vigna unguiculata Using CRISPR-Cas9
	Ji, Jie 1; Zhang, Chunyang 1; Sun, Zhongfeng 1; Wang, Longlong 1; Duanmu, Deqiang 1; Fan, Qiuling 2
通讯作者	Duanmu, Deqiang ; Fan, Qiuling
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
EISSN	1422-0067
出版年	2019
卷号	20 期号:10
英文摘要	Cowpea (Vigna unguiculata) is widely cultivated across the world. Due to its symbiotic nitrogen fixation capability and many agronomically important traits, such as tolerance to low rainfall and low fertilization requirements, as well as its high nutrition and health benefits, cowpea is an important legume crop, especially in many semi-arid countries. However, research in Vigna unguiculata is dramatically hampered by the lack of mutant resources and efficient tools for gene inactivation in vivo. In this study, we used clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9). We applied the CRISPR/Cas9-mediated genome editing technology to efficiently disrupt the representative symbiotic nitrogen fixation (SNF) gene in Vigna unguiculata. Our customized guide RNAs (gRNAs) targeting symbiosis receptor-like kinase (SYMRK) achieved similar to 67% mutagenic efficiency in hairy-root-transformed plants, and nodule formation was completely blocked in the mutants with both alleles disrupted. Various types of mutations were observed near the PAM region of the respective gRNA. These results demonstrate the applicability of the CRISPR/Cas9 system in Vigna unguiculata, and therefore should significantly stimulate functional genomics analyses of many important agronomical traits in this unique crop legume.
英文关键词	genome editing CRISPR Cas9 cowpea symbiosis nitrogen fixation
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	Green Published, gold, Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000471001400102
WOS关键词	AGROBACTERIUM-RHIZOGENES ; GENETIC-TRANSFORMATION ; WALP. ; ROOT ; MUTAGENESIS ; RHIZOBIUM ; PHASEOLUS ; DIVERSITY ; CPF1 ; CROP
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216470
作者单位	1.Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China; 2.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Ji, Jie,Zhang, Chunyang,Sun, Zhongfeng,et al. Genome Editing in Cowpea Vigna unguiculata Using CRISPR-Cas9[J],2019,20(10).
APA	Ji, Jie,Zhang, Chunyang,Sun, Zhongfeng,Wang, Longlong,Duanmu, Deqiang,&Fan, Qiuling.(2019).Genome Editing in Cowpea Vigna unguiculata Using CRISPR-Cas9.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(10).
MLA	Ji, Jie,et al."Genome Editing in Cowpea Vigna unguiculata Using CRISPR-Cas9".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.10(2019).