干旱区生态环境知识资源中心(Arid): Quantitative Proteomic Analysis of the Response to Cold Stress in Jojoba, a Tropical Woody Crop

Arid

DOI	10.3390/ijms20020243
	Quantitative Proteomic Analysis of the Response to Cold Stress in Jojoba, a Tropical Woody Crop
	Gao, Fei 1,2; Ma, Pengju 1; Wu, Yingxin 1; Zhou, Yijun 1; Zhang, Genfa 2,3
通讯作者	Gao, Fei ; Zhang, Genfa
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
EISSN	1422-0067
出版年	2019
卷号	20 期号:2
英文摘要	Jojoba (Simmondsia chinensis) is a semi-arid, oil-producing industrial crop that have been widely cultivated in tropical arid region. Low temperature is one of the major environmental stress that impair jojoba's growth, development and yield and limit introduction of jojoba in the vast temperate arid areas. To get insight into the molecular mechanisms of the cold stress response of jojoba, a combined physiological and quantitative proteomic analysis was conducted. Under cold stress, the photosynthesis was repressed, the level of malondialdehyde (MDA), relative electrolyte leakage (REL), soluble sugars, superoxide dismutase (SOD) and phenylalanine ammonia-lyase (PAL) were increased in jojoba leaves. Of the 2821 proteins whose abundance were determined, a total of 109 differentially accumulated proteins (DAPs) were found and quantitative real time PCR (qRT-PCR) analysis of the coding genes for 7 randomly selected DAPs were performed for validation. The identified DAPs were involved in various physiological processes. Functional classification analysis revealed that photosynthesis, adjustment of cytoskeleton and cell wall, lipid metabolism and transport, reactive oxygen species (ROS) scavenging and carbohydrate metabolism were closely associated with the cold stress response. Some cold-induced proteins, such as cold-regulated 47 (COR47), staurosporin and temperature sensitive 3-like a (STT3a), phytyl ester synthase 1 (PES1) and copper/zinc superoxide dismutase 1, might play important roles in cold acclimation in jojoba seedlings. Our work provided important data to understand the plant response to the cold stress in tropical woody crops.
英文关键词	Simmondsia chinensis cold stress proteomics leaf iTRAQ
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	gold, Green Published, Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000459746500011
WOS关键词	GENE-EXPRESSION ; CELL-WALL ; ENHANCES TOLERANCE ; NICOTIANA-TABACUM ; SUGAR METABOLISM ; ABSCISIC-ACID ; ARABIDOPSIS ; PLANT ; PROTEINS ; ACCLIMATION
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
来源机构	北京师范大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216468
作者单位	1.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China; 2.Beijing Key Lab Gene Resource & Mol Dev, Beijing 100875, Peoples R China; 3.Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China
推荐引用方式 GB/T 7714	Gao, Fei,Ma, Pengju,Wu, Yingxin,et al. Quantitative Proteomic Analysis of the Response to Cold Stress in Jojoba, a Tropical Woody Crop[J]. 北京师范大学,2019,20(2).
APA	Gao, Fei,Ma, Pengju,Wu, Yingxin,Zhou, Yijun,&Zhang, Genfa.(2019).Quantitative Proteomic Analysis of the Response to Cold Stress in Jojoba, a Tropical Woody Crop.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(2).
MLA	Gao, Fei,et al."Quantitative Proteomic Analysis of the Response to Cold Stress in Jojoba, a Tropical Woody Crop".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.2(2019).