干旱区生态环境知识资源中心(Arid): Macromolecular pullulan produced by Aureobasidium melanogenum 13-2 isolated from the Taklimakan desert and its crucial roles in resistance to the stress treatments

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DOI	10.1016/j.ijbiomac.2019.05.190
	Macromolecular pullulan produced by Aureobasidium melanogenum 13-2 isolated from the Taklimakan desert and its crucial roles in resistance to the stress treatments
	Jiang, Hong 1; Chen, Tie-Jun 1; Chi, Zhe 1,2; Hu, Zhong 3; Liu, Guang-Lei 1,2; Sun, Yi 1; Zhang, Shu-Hang 1; Chi, Zhen-Ming 1,2
通讯作者	Chi, Zhe
来源期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
EISSN	1879-0003
出版年	2019
卷号	135 页码:429-436
英文摘要	A novel yeast strain Aureobasidium melanogenum 13-2 isolated from the Taklimakan desert was found to be able to produce a high level of extracellular polysaccharide (EPS). Under the optimal conditions, the yeast strain could yield 73.25 +/- 2.3 g/L of EPS within 5 days at a flask level. During a 10-liter fermentation, the yeast strain could produce 78.05 +/- 3.5 g/L of EPS within 120 h. The FT-IR spectra of the standard pullulan from Sigma and the purified EPS produced by A. melanogenum 13-2 were almost identical and the purified EPS could be actively hydrolyzed by a pullulanase, demonstrating that the purified EPS was pullulan. The molecular weight (Mw) of the purified pullulan was estimated to be 7.703 x 10(5) g/moL. Disruption of a pullulan synthase gene (PUL1) made a mutant DAG27 lose the ability to synthesize any pullulan. The mutant DAG27 was more sensitive to radiation of UV light, high NaCl concentration, heat treatment, strong oxidation of H2O2 and desiccation than its wild type strain 13-2, suggesting that the produced pullulan could play an important role in resistance of the yeast cells to various stresses. This was the first time to show that the yeast strain obtained from the desert could produce such high level pullulan and the produced pullulan had an obviously protective effect on its producer. (C) 2019 Published by Elsevier B.V.
英文关键词	Aureobasidium melanogenum Exopolysaccharide Harsh environment Pullulan function The Taklimakan desert
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000477691600047
WOS关键词	MOLECULAR-WEIGHT ; EXOPOLYSACCHARIDE ; YEAST ; BIOSYNTHESIS ; SUCROSE ; STRAIN ; FERMENTATION ; OPTIMIZATION ; TREHALOSE ; GENETICS
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216357
作者单位	1.Ocean Univ China, Dept Biol, Coll Marine Life Sci, Yushan Rd 5, Qingdao, Shandong, Peoples R China; 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266003, Shandong, Peoples R China; 3.Shantou Univ, Dept Biol, Shantou 515063, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Hong,Chen, Tie-Jun,Chi, Zhe,et al. Macromolecular pullulan produced by Aureobasidium melanogenum 13-2 isolated from the Taklimakan desert and its crucial roles in resistance to the stress treatments[J],2019,135:429-436.
APA	Jiang, Hong.,Chen, Tie-Jun.,Chi, Zhe.,Hu, Zhong.,Liu, Guang-Lei.,...&Chi, Zhen-Ming.(2019).Macromolecular pullulan produced by Aureobasidium melanogenum 13-2 isolated from the Taklimakan desert and its crucial roles in resistance to the stress treatments.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,135,429-436.
MLA	Jiang, Hong,et al."Macromolecular pullulan produced by Aureobasidium melanogenum 13-2 isolated from the Taklimakan desert and its crucial roles in resistance to the stress treatments".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 135(2019):429-436.