干旱区生态环境知识资源中心(Arid): Design and evaluation of a hypoallergenic peptide-based vaccine for Salsola kali allergy

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DOI	10.1016/j.intimp.2018.10.037
	Design and evaluation of a hypoallergenic peptide-based vaccine for Salsola kali allergy
	Tabesh, Saeideh 1,2; Fanuel, Songwe 3,4; Fazlollahi, Mohammad Reza 2; Yekaninejad, Mir Saeed 5; Kardar, Gholam Ali 2,3; Razavi, Seyed Alireza 1
通讯作者	Razavi, Seyed Alireza
来源期刊	INTERNATIONAL IMMUNOPHARMACOLOGY
ISSN	1567-5769
EISSN	1878-1705
出版年	2019
卷号	66 页码:62-68
英文摘要	Background: The Salsola kali (S. kali) pollen is one of the most important causes of allergic rhinitis in the deserts and semi-desert areas. Immunotherapy with allergen extracts remains the only available treatment addressing the underlying mechanism of allergy. However, given the low efficacy of this method, it is necessary to find more effective and alternative therapeutic interventions using molecular biology and bioinformatics tools. In this study, a hypoallergenic vaccine was designed on the basis of B-cell epitope approach for S. kali immunotherapy. Methods: Using the Immune Epitope Database (IEDB), a 35-mer peptide was selected and chemically conjugated to a keyhole limpet hemocyanin (KLH) molecule. Specific IgG and IgE from immunized BALB/c mice sera against the vaccine (Salk 1-KLH), S. kali extract and the recombinant protein, rSal k 1, were measured using ELISA. Also, inhibition of IgE by mouse IgG was evaluated using an inhibitory ELISA. Finally, the IgE reactivity and T-cell reactivity of the designed vaccine were evaluated by dot blot assay and MTT assay. Results: Vaccination with the vaccine produced high levels of protective IgG in mice, which inhibited the binding of patients IgE to recombinant proteins. The result showed that the designed vaccine, unlike the recombinant protein and extract, did not induce T-cell lymphocytes response and also exhibited decreased IgE reactivity. Conclusion: The designed vaccine can be considered as a promising candidate for therapeutic allergen-specific immunotherapy.
英文关键词	Allergen Allergen-specific immunotherapy B-epitope Russian thistle Sal k 1
类型	Article
语种	英语
国家	Iran ; Zimbabwe
收录类别	SCI-E
WOS记录号	WOS:000456225700007
WOS关键词	ANTIHAPTEN IMMUNE-RESPONSES ; GRASS-POLLEN ALLERGEN ; B-CELL EPITOPES ; BASOPHIL ACTIVATION ; CARRIER FUNCTION ; IMMUNOTHERAPY ; MECHANISMS ; SENSITIZATION ; EFFICACY ; ISOFORM
WOS类目	Immunology ; Pharmacology & Pharmacy
WOS研究方向	Immunology ; Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216322
作者单位	1.Univ Tehran Med Sci, Sch Publ Hlth, Dept Immunol, Tehran, Iran; 2.Univ Tehran Med Sci, Immunol Asthma & Allergy Res Inst, Tehran, Iran; 3.Univ Tehran Med Sci, Sch Adv Technol Med, Dept Med Biotechnol, Tehran, Iran; 4.MSU, Fac Sci & Technol, Dept Appl Biosci & Biotechnol, Gweru, Zimbabwe; 5.Univ Tehran Med Sci, Sch Publ Hlth, Dept Epidemiol & Biostat, Tehran, Iran
推荐引用方式 GB/T 7714	Tabesh, Saeideh,Fanuel, Songwe,Fazlollahi, Mohammad Reza,et al. Design and evaluation of a hypoallergenic peptide-based vaccine for Salsola kali allergy[J],2019,66:62-68.
APA	Tabesh, Saeideh,Fanuel, Songwe,Fazlollahi, Mohammad Reza,Yekaninejad, Mir Saeed,Kardar, Gholam Ali,&Razavi, Seyed Alireza.(2019).Design and evaluation of a hypoallergenic peptide-based vaccine for Salsola kali allergy.INTERNATIONAL IMMUNOPHARMACOLOGY,66,62-68.
MLA	Tabesh, Saeideh,et al."Design and evaluation of a hypoallergenic peptide-based vaccine for Salsola kali allergy".INTERNATIONAL IMMUNOPHARMACOLOGY 66(2019):62-68.