干旱区生态环境知识资源中心(Arid): Nuclease activity decreases the RNAi response in the sweetpotato weevil Cylas puncticollis

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DOI	10.1016/j.ibmb.2019.04.001
	Nuclease activity decreases the RNAi response in the sweetpotato weevil Cylas puncticollis
	Prentice, Katterinne 1,2; Smagghe, Guy 1; Gheysen, Godelieve 2; Christians, Oliver 1
通讯作者	Christians, Oliver
来源期刊	INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
ISSN	0965-1748
EISSN	1879-0240
出版年	2019
卷号	110 页码:80-89
英文摘要	RNA interference (RNAi) refers to the process of suppression of gene expression in eukaryotes, which has a great potential for the control of pest and diseases. Unfortunately, the efficacy of this technology is limited or at best variable in some insects. In the African sweet potato weevil (SPW) Cylas puncticollis, a devastating pest that affects the sweet potato production in Sub-Saharan Africa (SSA), the RNAi response was highly efficient when dsRNA was delivered by injection, but it showed a reduced response by oral feeding. In the present study, the role of nucleases in the reduced RNAi efficiency in C. puncticollis is investigated. Several putative dsRNases were first identified in the transcriptome of the SPW through homology search and were subsequently further characterized. Two of these dsRNases were specifically expressed in the gut tissue of the insect and we could demonstrate through RNAi experiments that these affected dsRNA stability in the gut. Furthermore, RNAi-of-RNAi studies, using snf7 as a reporter gene, demonstrated that silencing one of these nucleases, Cp-dsRNase-3, clearly increases RNAi efficacy. After silencing this nuclease, significantly higher mortality was observed in dssnf7-treated insects 14 days post-feeding as compared to control treatments, and the gene downregulation was confirmed at the transcript level via qPCR analysis. Taken together, our results demonstrate that the RNAi efficiency is certainly impaired by nuclease activity in the gut environment of the SPW Cylas puncticollis.
英文关键词	Sweet potato weevil Cylas puncticollis RNA interference dsRNA degradation Nuclease
类型	Article
语种	英语
国家	Belgium
收录类别	SCI-E
WOS记录号	WOS:000472127200009
WOS关键词	DOUBLE-STRANDED-RNA ; DSRNA DEGRADATION ; DESERT LOCUST ; INTERFERENCE ; EXPRESSION ; BEETLE ; MICRORNAS ; PEST ; GUT ; EFFICIENCY
WOS类目	Biochemistry & Molecular Biology ; Entomology
WOS研究方向	Biochemistry & Molecular Biology ; Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216299
作者单位	1.Univ Ghent, Dept Plants & Crops, Fac Biosci Engn, B-9000 Ghent, Belgium; 2.Univ Ghent, Dept Biotechnol, Fac Biosci Engn, B-9000 Ghent, Belgium
推荐引用方式 GB/T 7714	Prentice, Katterinne,Smagghe, Guy,Gheysen, Godelieve,et al. Nuclease activity decreases the RNAi response in the sweetpotato weevil Cylas puncticollis[J],2019,110:80-89.
APA	Prentice, Katterinne,Smagghe, Guy,Gheysen, Godelieve,&Christians, Oliver.(2019).Nuclease activity decreases the RNAi response in the sweetpotato weevil Cylas puncticollis.INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY,110,80-89.
MLA	Prentice, Katterinne,et al."Nuclease activity decreases the RNAi response in the sweetpotato weevil Cylas puncticollis".INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 110(2019):80-89.