干旱区生态环境知识资源中心(Arid): Development of camelina enhanced with drought stress resistance and seed oil production by co-overexpression of MYB96A and DGAT1C

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DOI	10.1016/j.indcrop.2019.111475
	Development of camelina enhanced with drought stress resistance and seed oil production by co-overexpression of MYB96A and DGAT1C
	Kim, Ryeo Jin 1; Kim, Hyun Uk 2; Suh, Mi Chung 1
通讯作者	Kim, Hyun Uk ; Suh, Mi Chung
来源期刊	INDUSTRIAL CROPS AND PRODUCTS
ISSN	0926-6690
EISSN	1872-633X
出版年	2019
卷号	138
英文摘要	Camelina (Camelina sativa, Cs) is an emerging crop for the production of biodiesel and biofeedstock. This study aims to develop transgenic plants that have the advantage of two traits: improved drought resistance and oil content. Three genes, CsMYB96A, CsMYB96B, and CsMYB96C, were isolated from camelina stem. The deduced amino acid sequence of the three CsMYB96s showed at least 93% identity with Arabidopsis MYB96. CsMYB96A, B, and C transcripts were detected in various camelina tissues. Fluorescence signal from the fusion of CsMYB96A: enhanced yellow fluorescent protein was confined to the nucleus of tobacco epidermal cells. Transactivation analysis of tobacco protoplasts revealed that CsMYB96A was a transcription activator. Wax biosynthesis genes such as camelina beta-ketoacyl-CoA synthase 2, beta-ketoacyl-CoA synthase 6, beta-ketoacyl-CoA reductase 1-1, beta-ketoacylCoA reductase 1-2, enoyl-CoA reductase, ECERIFERUM 1 and ECERIFERUM 3 were upregulated approximately 2 to 120 times by CsMYB96A, indicating that CsMYB96A was involved in the activating of cuticular wax synthesis on plant epidemics. Camelina diacylglycerol acyltransferase 1C (CsDGAT1C) has been shown to increased oil synthesis genes in Arabidopsis. When CsDGAT1C and CsMYB96A were co-overexpressed in camelina, total fatty acid levels in transgenic seeds increased by approximately 21%. In addition, the transgenic camelina plants showed improved resistance to drought stress. This result suggests that the transgenic camelina can be grown to produce biodiesel and biofeedstock in arid or semi-arid lands.
英文关键词	Biodiesel Camelina Drought resistance DGAT1 MYB96 Seed oil
类型	Article
语种	英语
国家	South Korea
收录类别	SCI-E
WOS记录号	WOS:000484647800007
WOS关键词	CUTICULAR WAX BIOSYNTHESIS ; LIPID TRANSFER PROTEIN ; TRANSCRIPTION FACTORS ; OVER-EXPRESSION ; TRIACYLGLYCEROL BIOSYNTHESIS ; CUTICLE DEVELOPMENT ; ARABIDOPSIS ; GENE ; ACYLTRANSFERASE ; SATIVA
WOS类目	Agricultural Engineering ; Agronomy
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216295
作者单位	1.Sogang Univ, Dept Life Sci, Seoul 04107, South Korea; 2.Sejong Univ, Dept Bioind & Bioresource Engn, Plant Engn Res Inst, Seoul 05006, South Korea
推荐引用方式 GB/T 7714	Kim, Ryeo Jin,Kim, Hyun Uk,Suh, Mi Chung. Development of camelina enhanced with drought stress resistance and seed oil production by co-overexpression of MYB96A and DGAT1C[J],2019,138.
APA	Kim, Ryeo Jin,Kim, Hyun Uk,&Suh, Mi Chung.(2019).Development of camelina enhanced with drought stress resistance and seed oil production by co-overexpression of MYB96A and DGAT1C.INDUSTRIAL CROPS AND PRODUCTS,138.
MLA	Kim, Ryeo Jin,et al."Development of camelina enhanced with drought stress resistance and seed oil production by co-overexpression of MYB96A and DGAT1C".INDUSTRIAL CROPS AND PRODUCTS 138(2019).