干旱区生态环境知识资源中心(Arid): An efficient protocol for in vitro propagation of the wild legume Cicer microphyllum Benth., a crop wild relative of chickpea (Cicer arietinum L.)

Arid

DOI	10.1007/s11627-018-09958-y
	An efficient protocol for in vitro propagation of the wild legume Cicer microphyllum Benth., a crop wild relative of chickpea (Cicer arietinum L.)
	Singh, Rupesh Kumar 1; Anandhan, Sivalingam 2; Maria Garcia-Perez, Luz 3; Ruiz-May, Eliel 4; Nava Perez, Eusebio 3; Roberto Quiroz-Figueroa, Francisco 3
通讯作者	Roberto Quiroz-Figueroa, Francisco
来源期刊	IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
ISSN	1054-5476
EISSN	1475-2689
出版年	2019
卷号	55 期号:1 页码:9-14
英文摘要	Cicer microphyllum Benth. is a wild legume that is adapted to the extremely adverse climatic conditions of the cold Himalayan deserts. This rare species is a crop wild relative (CWR) that could be used for chickpea (Cicer arietinum L.) breeding programs to improve abiotic stress tolerance. However, the availability of C. microphyllum biological material is very limited due to its high altitude and remote location. These factors make in vitro plant tissue culture an excellent alternative for the conservation and propagation of this species. In this study, an efficient and reproducible in vitro regeneration protocol was established for C. microphyllum. This process enabled the regeneration of multiple shoots through indirect organogenesis from excised embryos. In addition, the effects of combinations of auxin and cytokinin concentrations in Murashige and Skoog (MS) medium were tested. The highest percentage of callus induction was observed on MS with -Naphthaleneacetic acid (NAA) and 6-Benzylaminopurine (BAP), and the addition of AgNO3 to MS with NAA and BAP significantly improved shoot morphogenesis. The highest root percentage was observed on half-strength MS with Indole-3-butyric acid (IBA). Regenerated plantlets had a 65% survival rate 25d after transplanting to garden soil, vermiculite, and vermicompost (1:1:1) potting mix. This protocol can be routinely used in large-scale propagation and possibly in germplasm conservation of this rare and important CWR species for additional use in chickpea breeding programs.
英文关键词	Shoot bud Micropropagation Plant tissue culture
类型	Article
语种	英语
国家	Portugal ; India ; Mexico
收录类别	SCI-E
WOS记录号	WOS:000460134100002
WOS关键词	REGENERATION ; STRESS ; MICROPROPAGATION ; PLANTS
WOS类目	Plant Sciences ; Cell Biology ; Developmental Biology
WOS研究方向	Plant Sciences ; Cell Biology ; Developmental Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216265
作者单位	1.Univ Tras Os Montes & Alto Douro, CQ VR, P-5000801 Vila Real, Portugal; 2.Directorate Onion & Garl Res, Pune 410505, Maharashtra, India; 3.IPN, CIIDIR, Unidad Sinaloa, Lab Fitomejoramiento Mol, Blvd Juan de Dios Batiz Paredes 250, Guasave 81101, Sinaloa, Mexico; 4.Inst Ecol AC, Red Estudios Mol Avanzados, Cluster BioMim, Carretera Antigua Coatepec 351, Xalapa 91070, Veracruz, Mexico
推荐引用方式 GB/T 7714	Singh, Rupesh Kumar,Anandhan, Sivalingam,Maria Garcia-Perez, Luz,et al. An efficient protocol for in vitro propagation of the wild legume Cicer microphyllum Benth., a crop wild relative of chickpea (Cicer arietinum L.)[J],2019,55(1):9-14.
APA	Singh, Rupesh Kumar,Anandhan, Sivalingam,Maria Garcia-Perez, Luz,Ruiz-May, Eliel,Nava Perez, Eusebio,&Roberto Quiroz-Figueroa, Francisco.(2019).An efficient protocol for in vitro propagation of the wild legume Cicer microphyllum Benth., a crop wild relative of chickpea (Cicer arietinum L.).IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,55(1),9-14.
MLA	Singh, Rupesh Kumar,et al."An efficient protocol for in vitro propagation of the wild legume Cicer microphyllum Benth., a crop wild relative of chickpea (Cicer arietinum L.)".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 55.1(2019):9-14.