干旱区生态环境知识资源中心(Arid): Molecular Existence and Diversity of Nitrite-Dependent Anaerobic Methane Oxidizing (n-Damo) Bacteria in the Lakes of Badain of the Gobi Desert

Arid

DOI	10.1080/01490451.2019.1578441
	Molecular Existence and Diversity of Nitrite-Dependent Anaerobic Methane Oxidizing (n-Damo) Bacteria in the Lakes of Badain of the Gobi Desert
	Zhang, Xiaowei 1; Liu, Kun 2,3; Li, Ping 4; Jiao, Jimmy J. J.3; Dvornyk, Volodymyr 1,5; Gu, Ji-Dong 1,6
通讯作者	Gu, Ji-Dong
来源期刊	GEOMICROBIOLOGY JOURNAL
ISSN	0149-0451
EISSN	1521-0529
出版年	2019
卷号	36 期号:6 页码:522-532
英文摘要	Nitrite-dependent anaerobic methane oxidation (n-damo) process, mediated by Candidatus Methylomirabilis oxyfera of the candidate phylum NC10, was discovered recently which plays an important role in coupling the global nitrogen and carbon cycles. However, the distribution and diversity of this new anaerobic methane-oxidizing microorganism have not been investigated in desert lakes yet. The present study successfully retrieved n-damo bacterial 16S rRNA and pmoA gene sequences using PCR technique from lakes in Badain Jaran Desert of China. Phylogenetic analyses showed that n-damo bacteria widely occurred in brine and freshwater lakes on the desert with high diversity, including both sediment and water samples. The results of quantitative PCR indicated that the abundance of the 16S rRNA gene in lake sediments varied from 1.12 +/- 0.68x10(5) to 1.64 +/- 0.70x10(5) copies g(-1) (dry weight), while that in water samples per milliliter was generally one order of magnitude lower than sediments. Correlation analyses suggested that n-damo bacterial abundance and diversity strongly depended on salinity. In lake sediments, the distribution, abundance, and diversity of n-damo bacteria were significantly associated with depth due to the concentration gradient of the NOx- and ammonium. This study provided new insights into both the n-damo community patterns and its interaction with ambient environmental factors in the desert lake ecosystem.
英文关键词	Abundance Badain Jaran Desert distribution diversity environmental factors nitrite-dependent anaerobic methane oxidation (n-damo)
类型	Article
语种	英语
国家	Peoples R China ; Saudi Arabia
收录类别	SCI-E
WOS记录号	WOS:000467885400005
WOS关键词	16S RIBOSOMAL-RNA ; METHANOTROPHIC BACTERIA ; ATMOSPHERIC METHANE ; OXIDATION BACTERIA ; SEDIMENTS ; HYPERSALINE ; COMMUNITY ; ALKALINE ; ARCHAEA ; SULFATE
WOS类目	Environmental Sciences ; Geosciences, Multidisciplinary
WOS研究方向	Environmental Sciences & Ecology ; Geology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215996
作者单位	1.Univ Hong Kong, Lab Environm Microbiol & Toxicol, Sch Biol Sci, Pokfulam Rd, Hong Kong, Peoples R China; 2.China Geol Survey, China Inst Geoenvironm Monitoring, Minist Nat Resources, Beijing, Peoples R China; 3.Univ Hong Kong, Dept Earth Sci, Hong Kong, Peoples R China; 4.China Univ Geosci, State Key Lab Biogeol & Environm Geol, Wuhan, Hubei, Peoples R China; 5.Alfaisal Univ, Dept Life Sci, Coll Sci & Gen Studies, Riyadh, Saudi Arabia; 6.City Univ Hong Kong, State Key Lab Marine Pollut, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiaowei,Liu, Kun,Li, Ping,et al. Molecular Existence and Diversity of Nitrite-Dependent Anaerobic Methane Oxidizing (n-Damo) Bacteria in the Lakes of Badain of the Gobi Desert[J],2019,36(6):522-532.
APA	Zhang, Xiaowei,Liu, Kun,Li, Ping,Jiao, Jimmy J. J.,Dvornyk, Volodymyr,&Gu, Ji-Dong.(2019).Molecular Existence and Diversity of Nitrite-Dependent Anaerobic Methane Oxidizing (n-Damo) Bacteria in the Lakes of Badain of the Gobi Desert.GEOMICROBIOLOGY JOURNAL,36(6),522-532.
MLA	Zhang, Xiaowei,et al."Molecular Existence and Diversity of Nitrite-Dependent Anaerobic Methane Oxidizing (n-Damo) Bacteria in the Lakes of Badain of the Gobi Desert".GEOMICROBIOLOGY JOURNAL 36.6(2019):522-532.