干旱区生态环境知识资源中心(Arid): Changes in Water Soluble Protein Profile of Antarctic Cyanobacterium Nostoc Commune under Temperature Downshift from 25 degrees to 5 degrees C

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DOI	10.1080/01490451.2018.1555293
	Changes in Water Soluble Protein Profile of Antarctic Cyanobacterium Nostoc Commune under Temperature Downshift from 25 degrees to 5 degrees C
	Tripathi, Ranjana; Rai, Pradeep Kumar; Rai, Anuradha; Singh, Surendra
通讯作者	Singh, Surendra
来源期刊	GEOMICROBIOLOGY JOURNAL
ISSN	0149-0451
EISSN	1521-0529
出版年	2019
卷号	36 期号:3 页码:271-277
英文摘要	The cyanobacterium Nostoc commune (Nostocales) is an isolate from the Schirmacher Oasis Antarctica. The cyanobacterium is psychrotropic in nature; and maintained in laboratory at 25 degrees C temperature, in unialgal form. Here, we studied the change in protein profile of water soluble proteins from exponentially growing N. commune upon downshift from its optimum growth temperature (25 degrees C) to a low temperature (5 degrees C). Experimental set up used to analyze the proteome were- a sudden shift to low temperature (i.e., cold shock), after short- (8 days) and long-term acclimation (7 weeks) to low temperature (5 degrees C). Cold-shock resulted in an increase in Low molecular weight proteins (LMWPs) with clouding of diffused proteins. Further increase in the duration of incubation period (short- and long-term acclimation) caused dissociation of proteins, indicated by NaCl (50-600mM) induced dissociation of proteins. That is, high molecular weight proteins (HMWPs) dissociated into LMWPs resulting in an increased number of protein bands. This was further confirmed by addition of LMWPs (<= 10KDa) resulting in re-association of proteins into HMWPs. Hence, we report that the cold-induced synthesis of LMWPs (<= 10kDa) is a strategy adopted by the N. commune to survive at low temperature of Antarctica.
英文关键词	Cold shock and acclimation cold stress proteins cyanobacteria LMWPs psychrotrophic
类型	Article
语种	英语
国家	India
收录类别	SCI-E
WOS记录号	WOS:000461644000009
WOS关键词	COLD SHOCK PROTEINS ; RNA-BINDING PROTEIN ; ACCLIMATION PROTEINS ; BACTERIUM ; IDENTIFICATION ; EXPRESSION ; INDUCTION
WOS类目	Environmental Sciences ; Geosciences, Multidisciplinary
WOS研究方向	Environmental Sciences & Ecology ; Geology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215994
作者单位	Banaras Hindu Univ, Ctr Adv Study Bot, Inst Sci, Varanasi, Uttar Pradesh, India
推荐引用方式 GB/T 7714	Tripathi, Ranjana,Rai, Pradeep Kumar,Rai, Anuradha,et al. Changes in Water Soluble Protein Profile of Antarctic Cyanobacterium Nostoc Commune under Temperature Downshift from 25 degrees to 5 degrees C[J],2019,36(3):271-277.
APA	Tripathi, Ranjana,Rai, Pradeep Kumar,Rai, Anuradha,&Singh, Surendra.(2019).Changes in Water Soluble Protein Profile of Antarctic Cyanobacterium Nostoc Commune under Temperature Downshift from 25 degrees to 5 degrees C.GEOMICROBIOLOGY JOURNAL,36(3),271-277.
MLA	Tripathi, Ranjana,et al."Changes in Water Soluble Protein Profile of Antarctic Cyanobacterium Nostoc Commune under Temperature Downshift from 25 degrees to 5 degrees C".GEOMICROBIOLOGY JOURNAL 36.3(2019):271-277.