干旱区生态环境知识资源中心(Arid): Genome-Wide Identification and Expression Analyses of the Chitinases under Cold and Osmotic Stress in Ammopiptanthus nanus

Arid

DOI	10.3390/genes10060472
	Genome-Wide Identification and Expression Analyses of the Chitinases under Cold and Osmotic Stress in Ammopiptanthus nanus
	Cao, Shilin 1; Wang, Ying 1; Li, Zhiqiang 1; Shi, Wei 2; Gao, Fei 1; Zhou, Yijun 1; Zhang, Genfa 3; Feng, Jinchao 1
通讯作者	Gao, Fei
来源期刊	GENES
EISSN	2073-4425
出版年	2019
卷号	10 期号:6
英文摘要	Chitinase is a kind of hydrolase with chitin as a substrate and is proposed to play an essential role in plant defense system by functioning against fungal pathogens through degrading chitin. Recent studies indicated chitinase is also involved in abiotic stress response in plants, helping plants to survive in stressful environments. A. nanus, a rare evergreen broad-leaved shrub distrusted in deserts in Central Asia, exhibits a high level of tolerance to drought and low temperature stresses. To identify the chitinase gene involved in drought and low temperature responses in A. nanus, we performed genome-wide identification, classification, sequence alignment, and spatio-temporal gene expression analysis of the chitinases in A. nanus under osmotic and low temperature stress. A total of 32 chitinase genes belonging to glycosyl hydrolase 18 (GH18) and GH19 families were identified from A. nanus. Class III chitinases appear to be amplified quantitatively in A. nanus, and their genes carry less introns, indicating their involvement in stress response in A. nanus. The expression level of the majority of chitinases varied in leaves, stems, and roots, and regulated under environmental stress. Some chitinases, such as EVM0022783, EVM0020238, and EVM0003645, are strongly induced by low temperature and osmotic stress, and the MYC/ICE1 (inducer of CBF expression 1) binding sites in promoter regions may mediate the induction of these chitinases under stress. These chitinases might play key roles in the tolerance to these abiotic stress in A. nanus and have potential for biotechnological applications. This study provided important data for understanding the biological functions of chitinases in A. nanus.
英文关键词	chitinase Ammopiptanthus nanus osmotic stress low temperature gene family
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	gold, Green Submitted, Green Published
收录类别	SCI-E
WOS记录号	WOS:000473797000067
WOS关键词	MULTIPLE SEQUENCE ALIGNMENT ; CLASS-III CHITINASE ; GENE FAMILY ; PLANT CHITINASES ; ARABIDOPSIS ; TRANSCRIPTION ; ANTIFREEZE ; ACTIVATION ; KINASE ; INDUCTION
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
来源机构	中国科学院新疆生态与地理研究所 ; 北京师范大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215872
作者单位	1.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China; 2.Chinese Acad Sci, Inst Ecol & Geog Xinjiang, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Xinjiang, Peoples R China; 3.Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China
推荐引用方式 GB/T 7714	Cao, Shilin,Wang, Ying,Li, Zhiqiang,et al. Genome-Wide Identification and Expression Analyses of the Chitinases under Cold and Osmotic Stress in Ammopiptanthus nanus[J]. 中国科学院新疆生态与地理研究所, 北京师范大学,2019,10(6).
APA	Cao, Shilin.,Wang, Ying.,Li, Zhiqiang.,Shi, Wei.,Gao, Fei.,...&Feng, Jinchao.(2019).Genome-Wide Identification and Expression Analyses of the Chitinases under Cold and Osmotic Stress in Ammopiptanthus nanus.GENES,10(6).
MLA	Cao, Shilin,et al."Genome-Wide Identification and Expression Analyses of the Chitinases under Cold and Osmotic Stress in Ammopiptanthus nanus".GENES 10.6(2019).