干旱区生态环境知识资源中心(Arid): De novo transcriptome assembly and co-expression network analysis of Cynanchum thesioides: Identification of genes involved in resistance to drought stress

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DOI	10.1016/j.gene.2019.05.055
	De novo transcriptome assembly and co-expression network analysis of Cynanchum thesioides: Identification of genes involved in resistance to drought stress
	Zhang, Xiaoyan 1; Yang, Zhongren 1; Li, Zhi 2; Zhang, Fenglan 1; Hao, Lizhen 1
通讯作者	Zhang, Fenglan
来源期刊	GENE
ISSN	0378-1119
EISSN	1879-0038
出版年	2019
卷号	710 页码:375-386
英文摘要	Cynanchum thesioides are upright, xerophytic shrubs that are widely distributed in arid and semi-arid areas of China, North Korea, Mongolia and Siberia. To date, little is known about the molecular mechanisms of drought resistance in C. thesioides. To better understand drought resistance, we used transcriptome analysis and Illumina sequencing technology on C. thesioides, to identify drought-responsive genes. Using de novo assembly 55,268 unigenes were identified from 207.58 Gb of clean data. Amongst these, 36,265 were annotated with gene descriptions, conserved domains, gene ontology terms and metabolic pathways. The sequencing results showed that genes that were differentially expressed (DEGs) under drought stress were enriched in pathways such as carbon metabolism, starch and sucrose metabolism, amino acid biosynthesis, phenylpropanoid biosynthesis and plant hormone signal transduction. Moreover, many functional genes were up-regulated under severe drought stress to enhance tolerance. Weighted gene co-expression network analysis showed that there were key hub genes related to drought stress. Hundreds of candidate genes were identified under severe drought stress, including transcriptional factors such as MYB, G2-like, ERF, C2H2, NAC, NF-X1, GRF, HD-ZIP, HB-other, HSF, C3H, GRAS, WRKY, bHLH and Trihelix. These data are a valuable resource for further investigation into the molecular mechanism for drought stress in C. thesioides and will facilitate exploration of drought resistance genes.
英文关键词	Cynanchum thesioides (Freyn) K. Schum. Cynanchum thesioides (Freyn) K. Schum. var. australe (maxim.) Tsiang et P. T. Li. Drought resistance Illumina sequencing Differentially expressed genes Weighted gene co-expression network
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000483423500045
WOS关键词	RESPONSES ; TOOL ; ANNOTATION
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215869
作者单位	1.Inner Mongolia Agr Univ, Coll Hort & Plant Protect, Inner Mongolia Key Lab Wild Peculiar Vegetable Ge, Hohhot 010019, Peoples R China; 2.Inner Mongolia Acad Agr & Anim Husb Sci, Special Crops Inst, Hohhot 010031, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiaoyan,Yang, Zhongren,Li, Zhi,et al. De novo transcriptome assembly and co-expression network analysis of Cynanchum thesioides: Identification of genes involved in resistance to drought stress[J],2019,710:375-386.
APA	Zhang, Xiaoyan,Yang, Zhongren,Li, Zhi,Zhang, Fenglan,&Hao, Lizhen.(2019).De novo transcriptome assembly and co-expression network analysis of Cynanchum thesioides: Identification of genes involved in resistance to drought stress.GENE,710,375-386.
MLA	Zhang, Xiaoyan,et al."De novo transcriptome assembly and co-expression network analysis of Cynanchum thesioides: Identification of genes involved in resistance to drought stress".GENE 710(2019):375-386.