干旱区生态环境知识资源中心(Arid): Molecular cloning and characterization of the beta-1,3-glucan recognition protein in Anatolica polita

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DOI	10.1016/j.gene.2019.02.043
	Molecular cloning and characterization of the beta-1,3-glucan recognition protein in Anatolica polita
	Yang, Xiaoxia 1; Mao, Xinfang 2; Xu, Xin 1; Li, Zaixin 2; Yang, Jianhua 3; Liu, Zhongyuan 2
通讯作者	Liu, Zhongyuan
来源期刊	GENE
ISSN	0378-1119
EISSN	1879-0038
出版年	2019
卷号	697 页码:144-151
英文摘要	beta-1,3-Glucan recognition protein (beta GRP) is an important pattern recognition protein, which could trigger immune response to eliminate pathogens by identifying and combining the pathogenic bacteria. In the present study, a beta-1,3-glucan recognition protein gene (Ap beta GRP) was cloned from a desert beetle Anatolica polita based on the EST sequence of Ap beta GRP in the suppression subtractive cDNA library. Quantitative real-time PCR (qRT-PCR) results showed that Ap beta GRP transcript in A. polita was significantly upregulated under the challenge of Escherichia coli and Staphylococcus aureus. Western blot analysis indicated that recombinant Ap beta GRP expressed in E. coli BL21, has the ability of binding to E. colt and S. aureus. Moreover, agglutination assay suggested that recombinant Ap beta GRP could agglutinate E. coli, S. aureus and Saccharomyces cerevisiae. The predicted 3D structure showed that Ap beta GRP possesses a typical beta-glucan recognition domain with seven beta-strands structures and conserved amino acid sequence. These data indicate that Ap beta GRP may be involved in immune defense in A. polita and could recognize and bind the bacteria against the invasion of external pathogens.
英文关键词	Anatolica polita beta-1,3-Glucan recognition protein Molecular cloning Microorganism binding Agglutination assay
类型	Article
语种	英语
国家	Peoples R China ; USA
收录类别	SCI-E
WOS记录号	WOS:000464481900018
WOS关键词	3-GLUCAN BINDING-PROTEIN ; PROPHENOLOXIDASE ACTIVATING SYSTEM ; CDNA CLONING ; MANDUCA-SEXTA ; LIPOPOLYSACCHARIDE ; BETA-1 ; PURIFICATION ; EXPRESSION ; LGBP ; BACTERIA
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
来源机构	新疆大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215868
作者单位	1.Xinjiang Univ, Xinjiang Key Lab Biol Resources & Genet Engn, Coll Life Sci & Technol, 14 Shengli Rd, Urumqi 830046, Peoples R China; 2.Sichuan Univ Sci & Engn, 180 Xueyuan St, Zigong 643000, Peoples R China; 3.Baylor Coll Med, Dept Pediat, Texas Childrens Canc Ctr, Houston, TX 77030 USA
推荐引用方式 GB/T 7714	Yang, Xiaoxia,Mao, Xinfang,Xu, Xin,et al. Molecular cloning and characterization of the beta-1,3-glucan recognition protein in Anatolica polita[J]. 新疆大学,2019,697:144-151.
APA	Yang, Xiaoxia,Mao, Xinfang,Xu, Xin,Li, Zaixin,Yang, Jianhua,&Liu, Zhongyuan.(2019).Molecular cloning and characterization of the beta-1,3-glucan recognition protein in Anatolica polita.GENE,697,144-151.
MLA	Yang, Xiaoxia,et al."Molecular cloning and characterization of the beta-1,3-glucan recognition protein in Anatolica polita".GENE 697(2019):144-151.