干旱区生态环境知识资源中心(Arid): Structural Characterization of ABCB1, the Gene Underlying the d2 Dwarf Phenotype in Pearl Millet, Cenchrus Americanus (L.) Morrone

Arid

DOI	10.1534/g3.118.200846
	Structural Characterization of ABCB1, the Gene Underlying the d2 Dwarf Phenotype in Pearl Millet, Cenchrus Americanus (L.) Morrone
	Parvathaneni, Rajiv K.1,4; Spiekerman, John J.2; Zhou, Hongye 3; Wu, Xiaomei 1,2,5; Devos, Katrien M.1,2
通讯作者	Devos, Katrien M.
来源期刊	G3-GENES GENOMES GENETICS
ISSN	2160-1836
出版年	2019
卷号	9 期号:8 页码:2497-2509
英文摘要	Pearl millet is an important food crop in arid and semi-arid regions of South Asia and sub-Saharan Africa and is grown in Australia and the United States as a summer fodder crop. The d2 dwarf germplasm has been widely used in the last half-century to develop high-performing pearl millet hybrids. We previously mapped the d2 phenotype to a 1.6 cM region in linkage group (LG) 4 and identified the ABCB1 gene as a candidate underlying the trait. Here, we report the sequence, structure and expression of ABCB1 in tall (D2D2) and d2 dwarf (d2d2) germplasm. The ABCB1 allele in d2 dwarfs differs from that in tall inbreds by the presence of two different high copy transposable elements, one in the coding region and the second located 664 bp upstream of the ATG start codon. These transposons were present in all d2 dwarfs tested that were reported to be of independent origin and absent in the analyzed wild-type tall germplasm. We also compared the expression profile of this gene in different organs of multiple tall and d2 dwarf inbreds, including the near-isogenic inbreds at the d2 locus, Tift 23B (D2D2) and Tift 23DB (d2d2). Heterologous transformation of the tall (Ca_ABCB1) and the d2 dwarf (Ca_abcb1) pearl millet alleles in the Arabidopsis double mutant abcb1abcb19 showed that the pearl millet D2 but not the d2 allele complements the Arabidopsis abcb1 mutation. Our studies also show the importance of the COOH-terminal 22 amino acids of the ABCB1 protein in either protein function or stability.
英文关键词	d2 dwarfing gene ABCB1 pearl millet LTR-retrotransposon heterologous transformation
类型	Article
语种	英语
国家	USA ; Peoples R China
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000479303300012
WOS关键词	AUXIN TRANSPORT ; FOXTAIL MILLET ; TWISTED DWARF1 ; DATABASE ; PROTEIN ; IDENTIFICATION ; TRANSFORMATION ; EXPRESSION ; MUTATIONS ; CANDIDATE
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215864
作者单位	1.Univ Georgia, Inst Plant Breeding Genet & Genom, Athens, GA 30602 USA; 2.Univ Georgia, Dept Plant Biol, Athens, GA 30602 USA; 3.Univ Georgia, Dept Genet, Athens, GA 30602 USA; 4.Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA; 5.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Parvathaneni, Rajiv K.,Spiekerman, John J.,Zhou, Hongye,et al. Structural Characterization of ABCB1, the Gene Underlying the d2 Dwarf Phenotype in Pearl Millet, Cenchrus Americanus (L.) Morrone[J],2019,9(8):2497-2509.
APA	Parvathaneni, Rajiv K.,Spiekerman, John J.,Zhou, Hongye,Wu, Xiaomei,&Devos, Katrien M..(2019).Structural Characterization of ABCB1, the Gene Underlying the d2 Dwarf Phenotype in Pearl Millet, Cenchrus Americanus (L.) Morrone.G3-GENES GENOMES GENETICS,9(8),2497-2509.
MLA	Parvathaneni, Rajiv K.,et al."Structural Characterization of ABCB1, the Gene Underlying the d2 Dwarf Phenotype in Pearl Millet, Cenchrus Americanus (L.) Morrone".G3-GENES GENOMES GENETICS 9.8(2019):2497-2509.