干旱区生态环境知识资源中心(Arid): Higher Expression Levels of Aquaporin Family of Proteins in the Kidneys of Arid-Desert Living Lepus yarkandensis

Arid

DOI	10.3389/fphys.2019.01172
	Higher Expression Levels of Aquaporin Family of Proteins in the Kidneys of Arid-Desert Living Lepus yarkandensis
	Zhang, Jianping 1,2,3; Li, Shuwei 1,3; Deng, Fang 1; Baikeli, Buheliqihan 1; Huang, Shuguang 2; Wang, Binyu 2; Liu, Guoquan 2,4
通讯作者	Zhang, Jianping ; Liu, Guoquan
来源期刊	FRONTIERS IN PHYSIOLOGY
ISSN	1664-042X
出版年	2019
卷号	10
英文摘要	Lepus yarkandensis specifically lives in arid climate with rare precipitation of Tarim Basin in western China. Aquaporins (AQPs) are a family of channel proteins that facilitate water transportation across cell membranes. Kidney AQPs play vital roles in renal tubule water permeability and maintenance of body water homeostasis. This study aimed to investigate whether kidney AQPs exhibit higher expression in arid-desert living animals. Immunohistochemistry results revealed localization of AQP1 to the capillary endothelial cells in glomerulus and epithelial cells in proximal tubule and descending thin limbs, AQP2 to the apical plasma membrane of principal cells in the cortical collecting duct (CCD), outer medullary collecting duct (OMCD), and IMCD cells in the initial inner medullary collecting duct (IMCD1) and middle IMCD (IMCD2), and AQP3 and AQP4 to the basolateral plasma membrane of principal cells and IMCD cells in CCD, OMCD, IMCD1, and IMCD2 in L. yarkandensis kidneys. Quantitative real-time PCR analysis showed higher mRNA levels of AQP1, AQP2, AQP3, and AQP4 in L. yarkandensis kidneys compared with Oryctolagus cuniculus. Similar results were obtained by western blotting. Our results suggested that higher expression levels of AQP1, AQP2, AQP3, and AQP4 in L. yarkandensis kidneys favored for drawing more water from the tubular fluid.
英文关键词	aquaporin family proteins kidneys water transport arid-desert living Lepus yarkandensis
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000485221100001
WOS关键词	WATER CHANNEL ; FREEZE TOLERANCE ; RENAL AQUAPORINS ; RAT-KIDNEY ; IMMUNOLOCALIZATION ; LOCALIZATION ; VASOPRESSIN ; PATHOPHYSIOLOGY ; PERMEABILITY ; DEHYDRATION
WOS类目	Physiology
WOS研究方向	Physiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215820
作者单位	1.Tarim Univ, Coll Life Sci, Alar, Peoples R China; 2.Huazhong Agr Univ, Coll Anim Sci & Vet Med, Dept Basic Vet Med, Wuhan, Hubei, Peoples R China; 3.Tarim Univ, Key Lab Protect & Utilizat Biol Resources Tarim B, Alar, Peoples R China; 4.Bengbu Med Coll, Dept Biochem, Coll Lab Med, Anhui Prov Key Lab Translat Canc Res, Bengbu, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jianping,Li, Shuwei,Deng, Fang,et al. Higher Expression Levels of Aquaporin Family of Proteins in the Kidneys of Arid-Desert Living Lepus yarkandensis[J],2019,10.
APA	Zhang, Jianping.,Li, Shuwei.,Deng, Fang.,Baikeli, Buheliqihan.,Huang, Shuguang.,...&Liu, Guoquan.(2019).Higher Expression Levels of Aquaporin Family of Proteins in the Kidneys of Arid-Desert Living Lepus yarkandensis.FRONTIERS IN PHYSIOLOGY,10.
MLA	Zhang, Jianping,et al."Higher Expression Levels of Aquaporin Family of Proteins in the Kidneys of Arid-Desert Living Lepus yarkandensis".FRONTIERS IN PHYSIOLOGY 10(2019).