干旱区生态环境知识资源中心(Arid): Phenotypic and Genotypic Diversity Among Symbiotic and Non-symbiotic Bacteria Present in Chickpea Nodules in Morocco

Arid

DOI	10.3389/fmicb.2019.01885
	Phenotypic and Genotypic Diversity Among Symbiotic and Non-symbiotic Bacteria Present in Chickpea Nodules in Morocco
	Benjelloun, Imane 1,2,3; Alami, Imane Thami 1; Douira, Allal 2; Udupa, Sripada M.3
通讯作者	Udupa, Sripada M.
来源期刊	FRONTIERS IN MICROBIOLOGY
EISSN	1664-302X
出版年	2019
卷号	10
英文摘要	Environmental pollution problems and increased demand for green technologies in production are forcing farmers to introduce agricultural practices with a lower impact on the environment. Chickpea (Cicer arietinum) in arid and semi-arid environments is frequently affected by harsh environmental stresses such as heat, drought and salinity, which limit its growth and productivity and affect biological nitrogen fixation ability of rhizobia. Climate change had further aggravated these stresses. Inoculation with appropriate stress tolerant rhizobia is necessary for an environmentally friendly and sustainable agricultural production. In this study, endophytic bacteria isolated from chickpea nodules from different soil types and regions in Morocco, were evaluated for their phenotypic and genotypic diversity in order to select the most tolerant ones for further inoculation of this crop. Phenotypic characterization of 135 endophytic bacteria from chickpea nodules showed a wide variability for tolerance to heavy metals and antibiotics, variable response to extreme temperatures, salinity, pH and water stress. 56% of isolates were able to nodulate chickpea. Numerical analysis of rep-PCR results showed that nodulating strains fell into 22 genotypes. Sequencing of 16S rRNA gene of endophytic bacteria from chickpea nodules revealed that 55% of isolated bacteria belong to Mesorhizobium genus. Based on MLSA of core genes (recA, atpD, glnll and dnaK), tasted strains were distributed into six clades and were closely related to Mesorhizobium ciceri, Mesorhizobium opportunistum, Mesorhizobium qingshengii, and Mesorhizobium plurifarium. Most of nodulating strains were belonging to a group genetically distinct from reference Mesorhizobium species. Three isolates belong to genus Burkholderia of the class beta- proteobacteria, and 55 other strains belong to the class gamma- proteobacteria. Some of the stress tolerant isolates have great potential for further inoculation of chickpea in the arid and semiarid environments to enhance biological nitrogen fixation and productivity in the context of climate change adaptation and mitigation.
英文关键词	rhizobia Cicer arietinum endophytic bacteria MLSA gamma proteobacteria beta proteobacteria
类型	Article
语种	英语
国家	Morocco
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000486382200001
WOS关键词	RHIZOBIA NODULATING CHICKPEA ; CICER-ARIETINUM L. ; GROWTH PROMOTING RHIZOBACTERIA ; MAJOR CHAPERONE GENES ; 16S RIBOSOMAL-RNA ; PLANT-GROWTH ; NITROGEN-FIXATION ; ENDOPHYTIC BACTERIA ; PHASEOLUS-VULGARIS ; MOLECULAR CHARACTERIZATION
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215810
作者单位	1.Natl Inst Agron Res, Dept Microbiol, Rabat, Morocco; 2.Ibn Tofail Univ, Fac Sci, Dept Biol, Kenitra, Morocco; 3.Int Ctr Agr Res Dry Areas, ICARDA INRA Cooperat Res Project, Rabat, Morocco
推荐引用方式 GB/T 7714	Benjelloun, Imane,Alami, Imane Thami,Douira, Allal,et al. Phenotypic and Genotypic Diversity Among Symbiotic and Non-symbiotic Bacteria Present in Chickpea Nodules in Morocco[J],2019,10.
APA	Benjelloun, Imane,Alami, Imane Thami,Douira, Allal,&Udupa, Sripada M..(2019).Phenotypic and Genotypic Diversity Among Symbiotic and Non-symbiotic Bacteria Present in Chickpea Nodules in Morocco.FRONTIERS IN MICROBIOLOGY,10.
MLA	Benjelloun, Imane,et al."Phenotypic and Genotypic Diversity Among Symbiotic and Non-symbiotic Bacteria Present in Chickpea Nodules in Morocco".FRONTIERS IN MICROBIOLOGY 10(2019).