干旱区生态环境知识资源中心(Arid): Actinobacteria and Cyanobacteria Diversity in Terrestrial Antarctic Microenvironments Evaluated by Culture-Dependent and Independent Methods

Arid

DOI	10.3389/fmicb.2019.01018
	Actinobacteria and Cyanobacteria Diversity in Terrestrial Antarctic Microenvironments Evaluated by Culture-Dependent and Independent Methods
	Rego, Adriana 1,2; Raio, Francisco 1; Martins, Teresa P.1; Ribeiro, Hugo 1,2; Sousa, Antonio G. G.1; Seneca, Joana 1; Baptista, Mafalda S.1,3; Lee, Charles K.3,4; Cary, S. Craig 3,4; Ramos, Vitor 1,6; Carvalho, Maria F.1; Leao, Pedro N.1; Magalhaes, Catarina 1,5
通讯作者	Magalhaes, Catarina
来源期刊	FRONTIERS IN MICROBIOLOGY
EISSN	1664-302X
出版年	2019
卷号	10
英文摘要	Bacterial diversity from McMurdo Dry Valleys in Antarctica, the coldest desert on earth, has become more easily assessed with the development of High Throughput Sequencing (HTS) techniques. However, some of the diversity remains inaccessible by the power of sequencing. In this study, we combine cultivation and HTS techniques to survey actinobacteria and cyanobacteria diversity along different soil and endolithic micro-environments of Victoria Valley in McMurdo Dry Valleys. Our results demonstrate that the Dry Valleys actinobacteria and cyanobacteria distribution is driven by environmental forces, in particular the effect of water availability and endolithic environments clearly conditioned the distribution of those communities. Data derived from HTS show that the percentage of cyanobacteria decreases from about 20% in the sample closest to the water source to negligible values on the last three samples of the transect with less water availability. Inversely, actinobacteria relative abundance increases from about 20% in wet soils to over 50% in the driest samples. Over 30% of the total HTS data set was composed of actinobacterial strains, mainly distributed by 5 families: Sporichthyaceae, Euzebyaceae, Patulibacteraceae, Nocardioidaceae, and Rubrobacteraceae. However, the 11 actinobacterial strains isolated in this study, belonged to Micrococcaceae and Dermacoccaceae families that were underrepresented in the HTS data set. A total of 10 cyanobacterial strains from the order Synechococcales were also isolated, distributed by 4 different genera (Nodosilinea, Leptolyngbya, Pectolyngbya, and Acaryochloris-like). In agreement with the cultivation results, Leptolyngbya was identified as dominant genus in the HTS data set. Acaryochloris-like cyanobacteria were found exclusively in the endolithic sample and represented 44% of the total 16S rRNA sequences, although despite our efforts we were not able to properly isolate any strain from this Acaryochloris-related group. The importance of combining cultivation and sequencing techniques is highlighted, as we have shown that culture-dependent methods employed in this study were able to retrieve actinobacteria and cyanobacteria taxa that were not detected in HTS data set, suggesting that the combination of both strategies can be useful' to recover both abundant and rare members of the communities.
英文关键词	actinobacteria McMurdo Dry Valleys Antarctic soil bacteria diversity bacterial cultivability endolitic microbiota Antarctic microenvironments cyanobacteria
类型	Article
语种	英语
国家	Portugal ; New Zealand
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000470126900001
WOS关键词	SOIL MICROBIAL COMMUNITIES ; MCMURDO DRY VALLEYS ; SP-NOV. ; ENDOLITHIC MICROORGANISMS ; PSYCHROPHILIC BACTERIUM ; FUNCTIONAL ECOLOGY ; WATER AVAILABILITY ; VICTORIA LAND ; COLONIZATION ; DESICCATION
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215797
作者单位	1.Univ Porto, Interdisciplinary Ctr Marine & Environm Res CIIMA, Porto, Portugal; 2.Univ Porto, Inst Biomed Sci Abel Salazar ICBAS, Porto, Portugal; 3.Univ Waikato, Int Ctr Terr Antarctic Res, Hamilton, New Zealand; 4.Univ Waikato, Sch Sci, Hamilton, New Zealand; 5.Univ Porto, Fac Sci, Porto, Portugal; 6.IPB, CIMO, Campus Santa Apolonia, Braganca, Portugal
推荐引用方式 GB/T 7714	Rego, Adriana,Raio, Francisco,Martins, Teresa P.,et al. Actinobacteria and Cyanobacteria Diversity in Terrestrial Antarctic Microenvironments Evaluated by Culture-Dependent and Independent Methods[J],2019,10.
APA	Rego, Adriana.,Raio, Francisco.,Martins, Teresa P..,Ribeiro, Hugo.,Sousa, Antonio G. G..,...&Magalhaes, Catarina.(2019).Actinobacteria and Cyanobacteria Diversity in Terrestrial Antarctic Microenvironments Evaluated by Culture-Dependent and Independent Methods.FRONTIERS IN MICROBIOLOGY,10.
MLA	Rego, Adriana,et al."Actinobacteria and Cyanobacteria Diversity in Terrestrial Antarctic Microenvironments Evaluated by Culture-Dependent and Independent Methods".FRONTIERS IN MICROBIOLOGY 10(2019).