干旱区生态环境知识资源中心(Arid): Comparison of Miniaturized Raman Spectrometers for Discrimination of Carotenoids of Halophilic Microorganisms

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DOI	10.3389/fmicb.2019.01155
	Comparison of Miniaturized Raman Spectrometers for Discrimination of Carotenoids of Halophilic Microorganisms
	Jehlicka, Jan 1; Culka, Adam 1; Mana, Lily 2; Oren, Aharon 2
通讯作者	Jehlicka, Jan
来源期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
出版年	2019
卷号	10
英文摘要	We present a comparison of the performance of four miniature portable Raman spectrometers for the discrimination of carotenoids in samples of carotene-producing microorganisms. Two spectrometers using a green laser allowing to obtain Resonance Raman (or pre-Resonance Raman) signals, one instrument with a 785 nm laser, and a recently developed Portable Sequentially Shifted Excitation Raman spectrometer (PSSERS) were used for identifying major pigments of different halophilic (genera Habbacterium, Halorubrum, Habarcula, Salinibacter, Ectothiorhodospira, Dunaliella) and non-halophilic microorganisms (Micrococcus luteus, Corynebacterium glutamicum). Using all the tested instruments including the PSSERS, strong carotenoids signals corresponding to the stretching vibrations in the polyene chain and in-plane rocking modes of the attached CH3 groups were found at the correct positions. Raman spectra of carotenoids can be obtained from different types of microbiological samples (wet pellets, lyophilized culture biomass and pigment extracts in organic solvents), and can be collected fast and without time-consuming procedures.
英文关键词	portable Raman spectrometers portable sequentially shifted excitation Raman spectrometer halophiles Salinibacter Corynebacterium Halorubrum Halobacterium exobiology
类型	Article
语种	英语
国家	Czech Republic ; Israel
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000469412100001
WOS关键词	VIBRATIONAL SPECTROSCOPY ; ATACAMA DESERT ; ARCHAEA ; CYANOBACTERIA ; EXCITATION ; SCYTONEMIN ; RADIATION ; BACTERIA ; PIGMENTS ; SPECTRA
WOS类目	Microbiology
WOS研究方向	Microbiology
来源机构	Hebrew University of Jerusalem
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215796
作者单位	1.Charles Univ Prague, Fac Sci, Inst Geochem Mineral & Mineral Resources, Prague, Czech Republic; 2.Hebrew Univ Jerusalem, Inst Life Sci, Jerusalem, Israel
推荐引用方式 GB/T 7714	Jehlicka, Jan,Culka, Adam,Mana, Lily,et al. Comparison of Miniaturized Raman Spectrometers for Discrimination of Carotenoids of Halophilic Microorganisms[J]. Hebrew University of Jerusalem,2019,10.
APA	Jehlicka, Jan,Culka, Adam,Mana, Lily,&Oren, Aharon.(2019).Comparison of Miniaturized Raman Spectrometers for Discrimination of Carotenoids of Halophilic Microorganisms.FRONTIERS IN MICROBIOLOGY,10.
MLA	Jehlicka, Jan,et al."Comparison of Miniaturized Raman Spectrometers for Discrimination of Carotenoids of Halophilic Microorganisms".FRONTIERS IN MICROBIOLOGY 10(2019).