干旱区生态环境知识资源中心(Arid): Nutrient Acquisition, Rather Than Stress Response Over Diel Cycles, Drives Microbial Transcription in a Hyper-Arid Namib Desert Soil

Arid

DOI	10.3389/fmicb.2019.01054
	Nutrient Acquisition, Rather Than Stress Response Over Diel Cycles, Drives Microbial Transcription in a Hyper-Arid Namib Desert Soil
	Leon-Sobrino, Carlos 1; Ramond, Jean-Baptiste 1; Maggs-Kolling, Gillian 2; Cowan, Don A.1
通讯作者	Cowan, Don A.
来源期刊	FRONTIERS IN MICROBIOLOGY
EISSN	1664-302X
出版年	2019
卷号	10
英文摘要	Hot desert surface soils are characterized by extremely low water activities for large parts of any annual cycle. It is widely assumed that microbial processes in such soils are very limited. Here we present the first metatranscriptomic survey of microbial community function in a low water activity hyperarid desert soil. Sequencing of total mRNA revealed a diverse and active community, dominated by Actinobacteria. Metatranscriptomic analysis of samples taken at different times over 3 days indicated that functional diel variations were limited at the whole community level, and mostly affected the eukaryotic subpopulation which was induced during the cooler night hours. High levels of transcription of chemoautotrophic carbon fixation genes contrasted with limited expression of photosynthetic genes, indicating that chemoautotrophy is an important alternative to photosynthesis for carbon cycling in desiccated desert soils. Analysis of the transcriptional levels of key N-cycling genes provided strong evidence that soil nitrate was the dominant nitrogen input source. Transcriptional network analyses and taxon-resolved functional profiling suggested that nutrient acquisition processes, and not diurnal environmental variation, were the main drivers of community activity in hyperarid Namib Desert soil. While we also observed significant levels of expression of common stress response genes, these genes were not dominant hubs in the co-occurrence network.
英文关键词	desert actinobacteria metatranscriptome analysis chemoautotrophic CO2 fixation diel activity cycles stress resistance and tolerance deserts and dryland ecosystems soil microbial activity RNA-seq
类型	Article
语种	英语
国家	South Africa ; Namibia
开放获取类型	Green Published, Green Submitted, gold
收录类别	SCI-E
WOS记录号	WOS:000467857400001
WOS关键词	DIFFERENTIAL EXPRESSION ANALYSIS ; CARBON-DIOXIDE FIXATION ; RNA-SEQ EXPERIMENTS ; GENE-EXPRESSION ; RIBOSOMAL-RNA ; BACTERIA ; COMMUNITIES ; DIVERSITY ; ECOLOGY ; AMPLIFICATION
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215793
作者单位	1.Univ Pretoria, Ctr Microbial Ecol & Genom, Pretoria, South Africa; 2.Gobabeb Res & Training Ctr, Gobabeb, Namibia
推荐引用方式 GB/T 7714	Leon-Sobrino, Carlos,Ramond, Jean-Baptiste,Maggs-Kolling, Gillian,et al. Nutrient Acquisition, Rather Than Stress Response Over Diel Cycles, Drives Microbial Transcription in a Hyper-Arid Namib Desert Soil[J],2019,10.
APA	Leon-Sobrino, Carlos,Ramond, Jean-Baptiste,Maggs-Kolling, Gillian,&Cowan, Don A..(2019).Nutrient Acquisition, Rather Than Stress Response Over Diel Cycles, Drives Microbial Transcription in a Hyper-Arid Namib Desert Soil.FRONTIERS IN MICROBIOLOGY,10.
MLA	Leon-Sobrino, Carlos,et al."Nutrient Acquisition, Rather Than Stress Response Over Diel Cycles, Drives Microbial Transcription in a Hyper-Arid Namib Desert Soil".FRONTIERS IN MICROBIOLOGY 10(2019).