干旱区生态环境知识资源中心(Arid): Plant Stage, Not Drought Stress, Determines the Effect of Cultivars on Bacterial Community Diversity in the Rhizosphere of Broomcorn Millet (Panicum miliaceum L.)

Arid

DOI	10.3389/fmicb.2019.00828
	Plant Stage, Not Drought Stress, Determines the Effect of Cultivars on Bacterial Community Diversity in the Rhizosphere of Broomcorn Millet (Panicum miliaceum L.)
	Na, Xiaofan 1; Cao, Xiaoning 2,3; Ma, Caixia 1; Ma, Shaolan 1; Xu, Pengxin 1; Liu, Sichen 2,3; Wang, Junjie 2,3; Wang, Haigang 2,3; Chen, Ling 2,3; Qiao, Zhijun 2,3
通讯作者	Na, Xiaofan ; Cao, Xiaoning
来源期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
出版年	2019
卷号	10
英文摘要	Broomcorn millet (Panicum miliaceum L.) is one of the oldest domesticated crops and has been grown in arid and semiarid areas in China since 10,000 cal. BP. However, limited information is available about how bacterial communities within the rhizosphere of different broomcorn millet cultivars respond to drought stress. Here, we characterized the changes in the rhizobacterial assemblages of two broomcorn millet cultivars, namely, P. miliaceum cv. HeQu Red (HQR) and P. miliaceum YanLi 10 (YL10), from the jointing stage to the grain filling stage after they were exposed to a short-term drought stress treatment at the seedling stage. Drought significantly inhibited the growth of both cultivars, but the effect on YL10 was higher than that on HQR, indicating that the drought tolerance of HQR was greater than that of YL10. Proteobacteria (33.8%), Actinobacteria (21.0%), Acidobacteria (10.7%), Bacteroidetes (8.2%), Chloroflexi (6.3%), Gemmatimonadetes (5.9%), Firmicutes (3.5%), Verrucomicrobia (2.9%), and Planctomycetes (2.7%) were the core bacterial components of broomcorn millet rhizosphere as suggested by 16S rDNA sequencing results. The diversity and composition of bacterial rhizosphere communities substantially varied at different developmental stages of broomcorn millet. As the plants matured, the richness and evenness of the rhizobacterial community significantly decreased. Principal coordinate analysis showed that the structure of the bacterial rhizosphere community changed notably only at the flowering stage between the two cultivars, suggesting a stage-dependent effect. Although drought stress had no significant effect on the diversity and structure of the bacterial rhizosphere community between the two cultivars, differential responses to drought was found in Actinobacteria and Acinetobacter, Lysobacter, Streptomyces, and Cellvibrio. The relative abundance of Actinobacteria and Lysobacter, Streptomyces, and Cellvibrio in the YL10 rhizosphere was stimulated by the drought treatment compared with that in the HQR rhizosphere, whereas the opposite effect was found in Acinetobacter. Our results suggested that the effects of cultivars on bacterial rhizosphere communities were highly dependent on plant developmental stage, reflecting the genetic variations in the two broomcorn millet cultivars.
英文关键词	broomcorn millet cultivar bacterial community rhizosphere drought stress plant age
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000465413800001
WOS关键词	GENETIC DIVERSITY ; RIBOSOMAL-RNA ; WATER-STRESS ; GROWTH ; AGE ; RHIZOBACTERIA ; TEMPERATURE ; MICROBIOME ; SENESCENCE ; IMPACT
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215789
作者单位	1.Ningxia Univ, Sch Life Sci, Yinchuan, Peoples R China; 2.Minist Agr, Key Lab Crop Gene Resources & Germplasm Enhancem, Taiyuan, Shanxi, Peoples R China; 3.Shanxi Acad Agr Sci, Inst Crop Germplasm Resources, Shanxi Key Lab Genet Resources & Genet Improvemen, Taiyuan, Shanxi, Peoples R China
推荐引用方式 GB/T 7714	Na, Xiaofan,Cao, Xiaoning,Ma, Caixia,et al. Plant Stage, Not Drought Stress, Determines the Effect of Cultivars on Bacterial Community Diversity in the Rhizosphere of Broomcorn Millet (Panicum miliaceum L.)[J],2019,10.
APA	Na, Xiaofan.,Cao, Xiaoning.,Ma, Caixia.,Ma, Shaolan.,Xu, Pengxin.,...&Qiao, Zhijun.(2019).Plant Stage, Not Drought Stress, Determines the Effect of Cultivars on Bacterial Community Diversity in the Rhizosphere of Broomcorn Millet (Panicum miliaceum L.).FRONTIERS IN MICROBIOLOGY,10.
MLA	Na, Xiaofan,et al."Plant Stage, Not Drought Stress, Determines the Effect of Cultivars on Bacterial Community Diversity in the Rhizosphere of Broomcorn Millet (Panicum miliaceum L.)".FRONTIERS IN MICROBIOLOGY 10(2019).