干旱区生态环境知识资源中心(Arid): Adaptive Strategies in a Poly-Extreme Environment: Differentiation of Vegetative Cells in Serratia ureilytica and Resistance to Extreme Conditions

Arid

DOI	10.3389/fmicb.2019.00102
	Adaptive Strategies in a Poly-Extreme Environment: Differentiation of Vegetative Cells in Serratia ureilytica and Resistance to Extreme Conditions
	Filippidou, Sevasti 1,2,3; Junier, Thomas 4; Wunderlin, Tina 1; Kooli, Wafa M.1; Palmieri, Ilona 1; Al-Dourobi, Andrej 1; Molina, Veronica 5; Lienhard, Reto 6; Spangenberg, Jorge E.7; Johnson, Shannon L.8; Chain, Patrick S. G.8; Dorador, Cristina 9,10; Junier, Pilar 1
通讯作者	Junier, Pilar
来源期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
出版年	2019
卷号	10
英文摘要	Poly-extreme terrestrial habitats are often used as analogs to extra-terrestrial environments. Understanding the adaptive strategies allowing bacteria to thrive and survive under these conditions could help in our quest for extra-terrestrial planets suitable for life and understanding how life evolved in the harsh early earth conditions. A prime example of such a survival strategy is the modification of vegetative cells into resistant resting structures. These differentiated cells are often observed in response to harsh environmental conditions. The environmental strain (strain Lr5/4) belonging to Serratia ureilytica was isolated from a geothermal spring in Lirima, Atacama Desert, Chile. The Atacama Desert is the driest habitat on Earth and furthermore, due to its high altitude, it is exposed to an increased amount of UV radiation. The geothermal spring from which the strain was isolated is oligotrophic and the temperature of 54 degrees C exceeds mesophilic conditions (15 to 45 degrees C). Although the vegetative cells were tolerant to various environmental insults (desiccation, extreme pH, glycerol), a modified cell type was formed in response to nutrient deprivation, UV radiation and thermal shock. Scanning (SEM) and Transmission Electron Microscopy (TEM) analyses of vegetative cells and the modified cell structures were performed. In SEM, a change toward a circular shape with reduced size was observed. These circular cells possessed what appears as extra coating layers under TEM. The resistance of the modified cells was also investigated, they were resistant to wet heat, UV radiation and desiccation, while vegetative cells did not withstand any of those conditions. A phylogenomic analysis was undertaken to investigate the presence of known genes involved in dormancy in other bacterial clades. Genes related to spore-formation in Myxococcus and Firmicutes were found in S. ureilytica Lr5/4 genome; however, these genes were not enough for a full sporulation pathway that resembles either group. Although, the molecular pathway of cell differentiation in S. ureilytica Lr5/4 is not fully defined, the identified genes may contribute to the modified phenotype in the Serratia genus. Here, we show that a modified cell structure can occur as a response to extremity in a species that was previously not known to deploy this strategy. This strategy may be widely spread in bacteria, but only expressed under poly-extreme environmental conditions.
英文关键词	differentiation dormancy extreme environments harsh conditions Serratia sporulation resistance
类型	Article
语种	英语
国家	Switzerland ; England ; Germany ; Chile ; USA
开放获取类型	Green Published, gold, Green Accepted
收录类别	SCI-E
WOS记录号	WOS:000457778400002
WOS关键词	MULTIPLE SEQUENCE ALIGNMENT ; BACILLUS-SUBTILIS ; RESTING FORMS ; MICROBIAL DIVERSITY ; MYXOCOCCUS-XANTHUS ; ARCHAEAL ISOLATE ; SP-NOV. ; SPORULATION ; ACID ; ENDOSPORES
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215780
作者单位	1.Univ Neuchatel, Lab Microbiol, Neuchatel, Switzerland; 2.Microbial Ecol Grp, Ctr Ecol & Hydrol, Wallingford, Oxon, England; 3.German Aerosp Ctr DLR eV, Inst Aerosp Med, Radiat Biol Dept, Space Microbiol Res Grp, Cologne, Germany; 4.Swiss Inst Bioinformat, Vital IT Grp, Lausanne, Switzerland; 5.Univ Playa Ancha, Fac Ciencias Nat & Exactas, Dept Biol, Valparaiso, Chile; 6.ADMED Microbiol, La Chaux De Fonds, Switzerland; 7.Univ Lausanne, Inst Earth Surface Dynam, Lausanne, Switzerland; 8.Los Alamos Natl Lab, Los Alamos, NM USA; 9.Univ Antofagasta, Fac Ciencias Mar & Recursos Biol, Dept Biotecnol, Lab Complejidad Microbiana & Ecol Func, Antofagasta, Chile; 10.Ctr Biotechnol & Bioengn, Antofagasta, Chile
推荐引用方式 GB/T 7714	Filippidou, Sevasti,Junier, Thomas,Wunderlin, Tina,et al. Adaptive Strategies in a Poly-Extreme Environment: Differentiation of Vegetative Cells in Serratia ureilytica and Resistance to Extreme Conditions[J],2019,10.
APA	Filippidou, Sevasti.,Junier, Thomas.,Wunderlin, Tina.,Kooli, Wafa M..,Palmieri, Ilona.,...&Junier, Pilar.(2019).Adaptive Strategies in a Poly-Extreme Environment: Differentiation of Vegetative Cells in Serratia ureilytica and Resistance to Extreme Conditions.FRONTIERS IN MICROBIOLOGY,10.
MLA	Filippidou, Sevasti,et al."Adaptive Strategies in a Poly-Extreme Environment: Differentiation of Vegetative Cells in Serratia ureilytica and Resistance to Extreme Conditions".FRONTIERS IN MICROBIOLOGY 10(2019).