干旱区生态环境知识资源中心(Arid): Soil Bacterial Communities From the Chilean Andean Highlands: Taxonomic Composition and Culturability

Arid

DOI	10.3389/fbioe.2019.00010
	Soil Bacterial Communities From the Chilean Andean Highlands: Taxonomic Composition and Culturability
	Maza, Felipe 1,2; Maldonado, Jonathan 1,2; Vasquez-Dean, Javiera 1; Mandakovic, Dinka 1,2; Gaete, Alexis 1,2; Cambiazo, Veronica 1,2; Gonzalez, Mauricio 1,2
通讯作者	Gonzalez, Mauricio
来源期刊	FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
ISSN	2296-4185
出版年	2019
卷号	7
英文摘要	The Atacama Desert is a highly complex, extreme ecosystem which harbors microorganisms remarkable for their biotechnological potential. Here, a soil bacterial prospection was carried out in the high Altiplano region of the Atacama Desert (>3,800 m above sea level; m a.s.I.), where direct anthropogenic interference is minimal. We studied: (1) soil bacterial community composition using high-throughput sequencing of the 16S rRNA gene and (2) bacterial culturability, by using a soil extract medium (SEM) under a factorial design of three factors: temperature (15 and 30 degrees C), nutrient content (high and low nutrient disposal) and oxygen availability (presence and absence). A total of 4,775 OTUs were identified and a total of 101 isolates were selected for 16S rRNA sequencing, 82 of them corresponded to unique or non-redundant sequences. To expand our view of the Altiplano landscape and to obtain a better representation of its microbiome, we complemented our Operational Taxonomic Units (OTUs) and isolate collection with data from other previous data from our group and obtained a merged set of OTUs and isolates that we used to perform our study. Taxonomic comparisons between culturable microbiota and metabarcoding data showed an overrepresentation of the phylum Firmicutes (44% of isolates vs. 2% of OTUs) and an underrepresentation of Proteobacteria (8% of isolates vs. 36% of OTUs). Within the Next Generation Sequencing (NGS) results, 33% of the OTUs were unknown up to genus, revealing an important proportion of putative new species in this environment. Biochemical characterization and analysis extracted from the literature indicated that an important number of our isolates had biotechnological potential. Also, by comparing our results with similar studies on other deserts, the Altiplano highland was most similar to a cold arid desert. In summary, our study contributes to expand the knowledge of soil bacterial communities in the Atacama Desert and complements the pipeline to isolate selective bacteria that could represent new potential biotechnological resources.
英文关键词	atacama desert altiplano highland bacterial community bacterial isolation NGS PGP
类型	Article
语种	英语
国家	Chile
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000458353500001
WOS关键词	MARS-LIKE SOILS ; ATACAMA DESERT ; ARID SOILS ; RHIZOSPHERIC BACTERIA ; MICROBIAL COMMUNITIES ; DIVERSITY ; GROWTH ; PUNA ; MICROORGANISMS ; LIFE
WOS类目	Biotechnology & Applied Microbiology ; Multidisciplinary Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215746
作者单位	1.Univ Chile, Inst Nutr & Tecnol Alimentos, Lab Bioinformat & Expres Gen, Santiago, Chile; 2.Ctr Genome Regulat, Santiago, Chile
推荐引用方式 GB/T 7714	Maza, Felipe,Maldonado, Jonathan,Vasquez-Dean, Javiera,et al. Soil Bacterial Communities From the Chilean Andean Highlands: Taxonomic Composition and Culturability[J],2019,7.
APA	Maza, Felipe.,Maldonado, Jonathan.,Vasquez-Dean, Javiera.,Mandakovic, Dinka.,Gaete, Alexis.,...&Gonzalez, Mauricio.(2019).Soil Bacterial Communities From the Chilean Andean Highlands: Taxonomic Composition and Culturability.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,7.
MLA	Maza, Felipe,et al."Soil Bacterial Communities From the Chilean Andean Highlands: Taxonomic Composition and Culturability".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 7(2019).