干旱区生态环境知识资源中心(Arid): Bioprospecting of Ureolytic Bacteria From Laguna Salada for Biomineralization Applications

Arid

DOI	10.3389/fbioe.2018.00209
	Bioprospecting of Ureolytic Bacteria From Laguna Salada for Biomineralization Applications
	Arias, Dayana 1,2; Cisternas, Luis A.1,3; Miranda, Carol 2; Rivas, Mariella 2,3
通讯作者	Rivas, Mariella
来源期刊	FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
ISSN	2296-4185
出版年	2019
卷号	6
英文摘要	The processes of biomineralization, mediated by ureolytic bacteria, possess a wide range of technological applications, such as the formation of biocements and remediation of water and soil environments. For this reason, the bioprospecting of new ureolytic bacteria is interesting for its application to these technologies, particularly for water treatment. This study demonstrates the isolation, selection, and identification of halotolerant ureolytic bacteria from Laguna Salada (inland from Atacama Desert) and the evaluation of their ability to precipitate calcium carbonate crystals in freshwater in the presence of calcium ions, as well as the ability to induce the precipitation of crystals from different ions present in seawater. Twenty-four halotolerant ureolytic bacteria whose molecular identification gives between 99 and 100% identity with species of the genus Bacillus, Porphyrobacter, Pseudomonas, Salinivibrio, and Halomonas were isolated. When cultivated in freshwater, urea, and calcium chloride, all species are able to biomineralize calcium carbonate in different concentrations. In seawater, the strains that biomineralize the highest concentration of calcium carbonate correspond to Bacillus subtilis and Halomonas sp. SEM-EDX and XRD analyses determined that both bacteria induce the formation of 9-33% halite (NaCl), 31-66% monohydrocalcite (CaCO3 x H2O), and 24-27% struvite (MgNH4PO4 x 6H(2)O). Additionally, B.subtilis induces the formation of 7% anhydrite (CaSO4). In seawater, B.subtilis and Halomonas sp. were able to precipitate both calcium (96-97%) and magnesium (63-67%) ions over 14 days of testing. Ion removal assays with B.subtilis immobilized in beads indicate a direct relationship between the urea concentration and a greater removal of ions with similar rates to free cells. These results demonstrate that the biomineralization mediated by bacterial urea hydrolysis is feasible in both freshwater and seawater, and we propose its application as a new technology in improving water quality for industrial uses.
英文关键词	biomineralization ureolytic bacteria struvite monohydrocalcite bioprospecting urease
类型	Article
语种	英语
国家	Chile
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000456157300001
WOS关键词	CALCIUM-CARBONATE ; UREASE ACTIVITY ; ENGINEERED APPLICATIONS ; STRUVITE PRECIPITATION ; LIQUID-MEDIA ; WASTE-WATER ; SEAWATER ; SOIL ; IMPROVEMENT ; PHOSPHATE
WOS类目	Biotechnology & Applied Microbiology ; Multidisciplinary Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215745
作者单位	1.Univ Antofagasta, Dept Ingn Quim & Proc Minerales, Antofagasta, Chile; 2.Univ Antofagasta, Lab Biotecnol Algal & Sustentabilidad BIOAL, Dept Biotecnol, Fac Ciencias Mar & Recursos Biol, Antofagasta, Chile; 3.Ctr Invest Cient Tecnol Mineria, Antofagasta, Chile
推荐引用方式 GB/T 7714	Arias, Dayana,Cisternas, Luis A.,Miranda, Carol,et al. Bioprospecting of Ureolytic Bacteria From Laguna Salada for Biomineralization Applications[J],2019,6.
APA	Arias, Dayana,Cisternas, Luis A.,Miranda, Carol,&Rivas, Mariella.(2019).Bioprospecting of Ureolytic Bacteria From Laguna Salada for Biomineralization Applications.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,6.
MLA	Arias, Dayana,et al."Bioprospecting of Ureolytic Bacteria From Laguna Salada for Biomineralization Applications".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 6(2019).