干旱区生态环境知识资源中心(Arid): Comparison of three different lipid removal cleanup techniques prior to the analysis of sulfonamide drug residues in porcine tissues

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DOI	10.1002/fsn3.1158
	Comparison of three different lipid removal cleanup techniques prior to the analysis of sulfonamide drug residues in porcine tissues
	Wang, Jie; Hu, Qiuhui; Li, Peng; Fang, Yong; Yang, Wenjian; Ma, Ning; Pei, Fei
通讯作者	Pei, Fei
来源期刊	FOOD SCIENCE & NUTRITION
ISSN	2048-7177
出版年	2019
卷号	7 期号:9 页码:3006-3016
英文摘要	A number of 17 sulfonamides (SNs) determination in porcine tissues using two new materials including Enhanced Matrix Removal for Lipid (EMR-L) and Oasis PRiME hydrophilic-lipophilic balance (HLB), and the conventional liquid-liquid extraction with n-hexane (LLE) sample preparation methods were evaluated and compared. Samples were extracted uniformly with acidified acetonitrile and cleaned up by the three sample preparation methods, and then, analytes were further separated by ultrahigh-performance liquid chromatography (UHPLC) and detected by a triple quadrupole mass spectrometry (MS/MS) or a quadrupole-time-of-flight tandem mass spectrometry (Q-TOF/MS). The results showed that the matrix effects from the EMR-L and HLB were significantly lower than that from LLE, suggesting that these two new materials are superior to n-hexane in the precipitation of proteins and the adsorption of lipids. Moreover, the recoveries of 17 SNs were quantified by the matrix-matched calibration curve at spiked level of 5, 10, and 20 mu g/kg, and 97.0% of the results satisfied method validation requirements. The samples cleaned up by EMR-L and HLB achieved the highest average recovery in liver and kidney with high moisture content, and muscle which is high in fat, respectively. In addition, Q-TOF/MS could play a good role in aided verification based on the result of repeated validation test. In summary, either combination of approaches could be used to achieve monitoring purposes; it is still worthwhile to adopt a specific sample preparation method and MS detector for the quantification in a specific matrix.
英文关键词	enhanced matrix removal for lipid hydrophilic-lipophilic balance mass spectrometry porcine tissues sulfonamides
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000480203500001
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; MULTICLASS VETERINARY DRUGS ; TANDEM MASS-SPECTROMETRY ; SAMPLE PREPARATION METHODS ; ANTIBIOTIC-RESIDUES ; PESTICIDE-RESIDUES ; TRIPLE QUADRUPOLE ; RAW-MILK ; EXTRACTION ; VALIDATION
WOS类目	Food Science & Technology
WOS研究方向	Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215663
作者单位	Nanjing Univ Finance & Econ, Coll Food Sci & Engn, Nanjing 210023, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jie,Hu, Qiuhui,Li, Peng,et al. Comparison of three different lipid removal cleanup techniques prior to the analysis of sulfonamide drug residues in porcine tissues[J],2019,7(9):3006-3016.
APA	Wang, Jie.,Hu, Qiuhui.,Li, Peng.,Fang, Yong.,Yang, Wenjian.,...&Pei, Fei.(2019).Comparison of three different lipid removal cleanup techniques prior to the analysis of sulfonamide drug residues in porcine tissues.FOOD SCIENCE & NUTRITION,7(9),3006-3016.
MLA	Wang, Jie,et al."Comparison of three different lipid removal cleanup techniques prior to the analysis of sulfonamide drug residues in porcine tissues".FOOD SCIENCE & NUTRITION 7.9(2019):3006-3016.