干旱区生态环境知识资源中心(Arid): Harnessing long-read amplicon sequencing to uncover NRPS and Type I PKS gene sequence diversity in polar desert soils

Arid

DOI	10.1093/femsec/fiz031
	Harnessing long-read amplicon sequencing to uncover NRPS and Type I PKS gene sequence diversity in polar desert soils
	Benaud, Nicole 1; Zhang, Eden 1; van Dorst, Josie 1; Brown, Mark V.2; Kalaitzis, John A.1; Neilan, Brett A.2; Ferrari, Belinda C.1
通讯作者	Ferrari, Belinda C.
来源期刊	FEMS MICROBIOLOGY ECOLOGY
ISSN	0168-6496
EISSN	1574-6941
出版年	2019
卷号	95 期号:4
英文摘要	The severity of environmental conditions at Earth's frigid zones present attractive opportunities for microbial biomining due to their heightened potential as reservoirs for novel secondary metabolites. Arid soil microbiomes within the Antarctic and Arctic circles are remarkably rich in Actinobacteria and Proteobacteria, bacterial phyla known to be prolific producers of natural products. Yet the diversity of secondary metabolite genes within these cold, extreme environments remain largely unknown. Here, we employed amplicon sequencing using PacBio RS II, a third generation long-read platform, to survey over 200 soils spanning twelve east Antarctic and high Arctic sites for natural product-encoding genes, specifically targeting non-ribosomal peptides (NRPS) and Type I polyketides (PKS). NRPS-encoding genes were more widespread across the Antarctic, whereas PKS genes were only recoverable from a handful of sites. Many recovered sequences were deemed novel due to their low amino acid sequence similarity to known protein sequences, particularly throughout the east Antarctic sites. Phylogenetic analysis revealed that a high proportion were most similar to antifungal and biosurfactant-type clusters. Multivariate analysis showed that soil fertility factors of carbon, nitrogen and moisture displayed significant negative relationships with natural product gene richness. Our combined results suggest that secondary metabolite production is likely to play an important physiological component of survival for microorganisms inhabiting arid, nutrient-starved soils.
英文关键词	polar soil microbial biodiversity amplicon sequencing polyketides non-ribosomal peptides
类型	Article
语种	英语
国家	Australia
收录类别	SCI-E
WOS记录号	WOS:000474761500006
WOS关键词	NATURAL-PRODUCTS ; NONRIBOSOMAL PEPTIDE ; COMMUNITY STRUCTURE ; BIOSYNTHESIS ; SURFACTANTS ; DISCOVERY
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215622
作者单位	1.UNSW, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia; 2.Univ Newcastle, Sch Environm & Life Sci, Callaghan, NSW 2308, Australia
推荐引用方式 GB/T 7714	Benaud, Nicole,Zhang, Eden,van Dorst, Josie,et al. Harnessing long-read amplicon sequencing to uncover NRPS and Type I PKS gene sequence diversity in polar desert soils[J],2019,95(4).
APA	Benaud, Nicole.,Zhang, Eden.,van Dorst, Josie.,Brown, Mark V..,Kalaitzis, John A..,...&Ferrari, Belinda C..(2019).Harnessing long-read amplicon sequencing to uncover NRPS and Type I PKS gene sequence diversity in polar desert soils.FEMS MICROBIOLOGY ECOLOGY,95(4).
MLA	Benaud, Nicole,et al."Harnessing long-read amplicon sequencing to uncover NRPS and Type I PKS gene sequence diversity in polar desert soils".FEMS MICROBIOLOGY ECOLOGY 95.4(2019).