干旱区生态环境知识资源中心(Arid): Proteomics and bioinformatics approaches for the identification of plasma biomarkers to detect Parkinson's disease

Arid

DOI	10.3892/etm.2019.7888
	Proteomics and bioinformatics approaches for the identification of plasma biomarkers to detect Parkinson's disease
	Dong, Wenwen 1; Qiu, Chang 1; Gong, Dawei 1; Jiang, Xu 2; Liu, Wan 2; Liu, Weiguo 2; Zhang, Li 2; Zhang, Wenbin 1
通讯作者	Zhang, Li ; Zhang, Wenbin
来源期刊	EXPERIMENTAL AND THERAPEUTIC MEDICINE
ISSN	1792-0981
EISSN	1792-1015
出版年	2019
卷号	18 期号:4 页码:2833-2842
英文摘要	The aim of the present study was to screen for biomarkers of Parkinson's disease (PD) using proteomics and bioinformatics approaches. PD patients were divided into three groups: Those without surgery (PD1 group); those who had undergone deep brain stimulation (DBS) surgery without electrode stimulation (PD2 group); and those who had undergone DBS surgery with 1 month of electrode stimulation (PD3 group). The non-Parkinson control group (CK group) was also involved. Quantitative proteomic analysis of human sera was performed through the use of tandem mass tag markers and liquid chromatography-mass spectrometry (LC-MS)-based techniques. For the proteins with quantitative information, a systematic bioinformatics analysis was then performed, including protein annotation, functional classification, functional enrichment and cluster analysis based on functional enrichment. Of the 739 proteins identified, quantitative information was available for 644. With regard to differential expression, 18 upregulated and 21 downregulated proteins were screened in the PD1/CK comparison group; 12 upregulated and 12 downregulated proteins in the PD2/PD1 comparison group; and 16 upregulated and 19 downregulated proteins in the PD3/PD2 comparison group. Coiled-coil domain-containing protein 154 (CCDC154) and tripartite motif-containing protein 3 (TRIM3) were key proteins involved in the molecular mechanisms of PD, participating in intracellular vesicle, ubiquitin protein ligase and transition metal ion-binding activities. After DBS surgery, desert hedgehog protein (DHH) was downregulated, whereas neuropilin-2 (NRP2) was upregulated; these participated in the ensheathment of neurons and the semaphorin receptor complex, respectively. The expression level of chloride intracellular channel protein 1 (CLIC1) was increased after 1 month of electrode stimulation following DBS. By combining proteomic approaches and LC-MS methods, significant proteins including CCDC154, TRIM3, DHH, NRP2 and CLIC1 were detected with high specificity and sensitivity. These may be used as novel biomarkers for early diagnosis of PD and the future development of treatments.
英文关键词	proteomics biomarker Parkinson's disease functional enrichment analysis cluster analysis
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	gold, Green Submitted, Green Published
收录类别	SCI-E
WOS记录号	WOS:000489717700060
WOS关键词	E3 UBIQUITIN LIGASE ; NEUROPROTEOMICS ; PATHOGENESIS ; DISORDERS ; DEMENTIA ; ETIOLOGY ; NEURONS
WOS类目	Medicine, Research & Experimental
WOS研究方向	Research & Experimental Medicine
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215614
作者单位	1.Nanjing Med Univ, Nanjing Brain Hosp, Dept Funct Neurosurg, 264 Guangzhou Rd, Nanjing 210029, Jiangsu, Peoples R China; 2.Nanjing Med Univ, Nanjing Brain Hosp, Dept Neurol, 264 Guangzhou Rd, Nanjing 210029, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Dong, Wenwen,Qiu, Chang,Gong, Dawei,et al. Proteomics and bioinformatics approaches for the identification of plasma biomarkers to detect Parkinson's disease[J],2019,18(4):2833-2842.
APA	Dong, Wenwen.,Qiu, Chang.,Gong, Dawei.,Jiang, Xu.,Liu, Wan.,...&Zhang, Wenbin.(2019).Proteomics and bioinformatics approaches for the identification of plasma biomarkers to detect Parkinson's disease.EXPERIMENTAL AND THERAPEUTIC MEDICINE,18(4),2833-2842.
MLA	Dong, Wenwen,et al."Proteomics and bioinformatics approaches for the identification of plasma biomarkers to detect Parkinson's disease".EXPERIMENTAL AND THERAPEUTIC MEDICINE 18.4(2019):2833-2842.