干旱区生态环境知识资源中心(Arid): Enantioselective determination of cathinones in urine by high pressure in-line SPE-CE

Arid

DOI	10.1002/elps.201900072
	Enantioselective determination of cathinones in urine by high pressure in-line SPE-CE
	Perez-Alcaraz, Albert; Borrull, Francesc; Aguilar, Carme; Calull, Marta
通讯作者	Aguilar, Carme
来源期刊	ELECTROPHORESIS
ISSN	0173-0835
EISSN	1522-2683
出版年	2019
卷号	40 期号:14 页码:1762-1770
英文摘要	This work presents a strategy based on the in-line coupling of SPE and CE for the chiral determination of cathinones (R,S-mephedrone, R,S-4-methylephedrine, and R,S- methylenedioxypyrovalerone) in urine samples, using a sample pretreatment based on liquid-liquid extraction. The chiral separation of the compounds is achieved by adding a mixture of 8 mM 2-hydroxypropil beta-CD and 5 mM beta-CD to the BGE, which consists of 70 mM of monosodium phosphate aqueous solution at pH 2.5. Oasis HLB was the selected sorbent for the in-line SPE device, and to reduce analysis time and LODs, several parameters affecting the in-line SPE system were evaluated, such as pressure and time of sample injection and dimensions of the SPE device. The highest preconcentration factors were achieved by using 3 bar of injection pressure for 20 min with an in-line SPE device of 2 mm length and 150 mu m of i.d. The developed method was applied to determine the presence of the compounds in spiked urine samples. The LODs obtained were between 3 and 8 ng/mL, and these levels were below the usual concentrations at which these drugs are present in urine from cathinone abusers. Thus, the optimized method has the potential to be applied for toxicological and forensic purposes.
英文关键词	Cathinones Chiral CE In-line solid phase extraction Oasis HLB Urine analysis
类型	Article
语种	英语
国家	Spain
收录类别	SCI-E
WOS记录号	WOS:000475494600002
WOS关键词	SOLID-PHASE EXTRACTION ; CAPILLARY-ELECTROPHORESIS ; LIQUID-CHROMATOGRAPHY ; SYNTHETIC CATHINONES ; MASS-SPECTROMETRY ; CHIRAL SEPARATION ; WATER SAMPLES ; DRUGS ; QUANTIFICATION ; IDENTIFICATION
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215340
作者单位	Univ Rovira & Virgili, Dept Analyt Chem & Organ Chem, Campus Sescelades,Marcel Li Domingo 1, E-43007 Tarragona, Spain
推荐引用方式 GB/T 7714	Perez-Alcaraz, Albert,Borrull, Francesc,Aguilar, Carme,et al. Enantioselective determination of cathinones in urine by high pressure in-line SPE-CE[J],2019,40(14):1762-1770.
APA	Perez-Alcaraz, Albert,Borrull, Francesc,Aguilar, Carme,&Calull, Marta.(2019).Enantioselective determination of cathinones in urine by high pressure in-line SPE-CE.ELECTROPHORESIS,40(14),1762-1770.
MLA	Perez-Alcaraz, Albert,et al."Enantioselective determination of cathinones in urine by high pressure in-line SPE-CE".ELECTROPHORESIS 40.14(2019):1762-1770.