干旱区生态环境知识资源中心(Arid): Entomopathogenic efficacy of the chitinolytic bacteria: Aeromonas hydrophila isolated from Siwa Oasis, Egypt

Arid

DOI	10.1186/s41938-019-0116-x
	Entomopathogenic efficacy of the chitinolytic bacteria: Aeromonas hydrophila isolated from Siwa Oasis, Egypt
	Korany, Shereen M.1; Mansour, Amany N.2; El-Hendawy, Hoda H.1; Kobisi, Abdel Naser A.2; Aly, Hamdy H.2
通讯作者	El-Hendawy, Hoda H.
来源期刊	EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
ISSN	1110-1768
EISSN	2536-9342
出版年	2019
卷号	29
英文摘要	Thirty-four bacterial isolates were isolated from soil samples collected from the North Western Coast and a water sample collected from brackish water at Siwa Oasis, Matrouh Governorate, Egypt. Only six isolates showed chitinase activity when screened on colloidal chitin agar medium. The highest chitinolytic activity was achieved by a bacterial isolate labeled as A.S. This isolate was identified as Aeromonas hydrophila based on analysis of 16S rRNA gene sequence and morphological, physiological, and biochemical characteristics. Optimization of the cultural conditions for maximum chitinase production by A. hydrophila revealed that the highest level of chitinase was recorded when the bacterium was grown in malt nitrogen-based medium containing 1% colloidal chitin at pH7 for 48h incubation at 30 degrees C. Crude chitinase from isolate A. hydrophila was evaluated against first instar larvae of the greater wax moth; Galleria mellonella L. (Lepidoptera: Pyralidae) at different concentrations of 0, 185, 205, 235, 265, 295U/mg protein. It increased larval and pupal mortality rates in a concentration-dependent manner. The tested crude chitinase significantly induced a decrease in adults' emergence rate and their fecundity.
英文关键词	Entomopathogens Bacteria Chitinase Aeromonas hydrophila Galleria mellonella
类型	Article
语种	英语
国家	Egypt
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000462303000001
WOS关键词	MARCESCENS STRAIN SEN ; SPODOPTERA-LITURA ; CHITINASE ; IDENTIFICATION ; OPTIMIZATION ; GROWTH ; STATE ; MOTH
WOS类目	Entomology
WOS研究方向	Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215335
作者单位	1.Helwan Univ, Fac Sci, Bot & Microbiol Dept, Cairo, Egypt; 2.Desert Res Ctr, Dept Plant Protect, Cairo, Egypt
推荐引用方式 GB/T 7714	Korany, Shereen M.,Mansour, Amany N.,El-Hendawy, Hoda H.,et al. Entomopathogenic efficacy of the chitinolytic bacteria: Aeromonas hydrophila isolated from Siwa Oasis, Egypt[J],2019,29.
APA	Korany, Shereen M.,Mansour, Amany N.,El-Hendawy, Hoda H.,Kobisi, Abdel Naser A.,&Aly, Hamdy H..(2019).Entomopathogenic efficacy of the chitinolytic bacteria: Aeromonas hydrophila isolated from Siwa Oasis, Egypt.EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL,29.
MLA	Korany, Shereen M.,et al."Entomopathogenic efficacy of the chitinolytic bacteria: Aeromonas hydrophila isolated from Siwa Oasis, Egypt".EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL 29(2019).