干旱区生态环境知识资源中心(Arid): High-resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chile

Arid

DOI	10.1002/ece3.5230
	High-resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chile
	Anabalon, Leonardo 1; Encina-Montoya, Francisco 2,3; Sanchez, Pamela 2; Solano, Jaime 4; Benavente, Felipe 5; Guinez, Basilio 6; Olivares, Flavio ; Oberti, Carlos 2; Vega, Rolando 4,7
通讯作者	Anabalon, Leonardo
来源期刊	ECOLOGY AND EVOLUTION
ISSN	2045-7758
出版年	2019
卷号	9 期号:13 页码:7448-7454
英文摘要	Easy, economic, precise species authentication is currently necessary in many areas of research and diagnosis in molecular biology applied to conservation studies of endangered species. Here, we present a new method for the identification of three fox species of the Lycalopex genus in Chile. We developed an assay based on high-resolution melt analysis of the mitochondrial cytochrome B gene, allowing a simple, low cost, fast, and accurate species determination. To validate the assay applicability for noninvasive samples, we collected fecal samples in the Atacama Desert, finding unexpectedly one species outside of its known distribution range. We conclude that the assay has a potential to become a valuable tool for a standardized genetic monitoring of the Lycalopex species in Chile.
英文关键词	barcode HRM Lycalopex PCR
类型	Article
语种	英语
国家	Chile
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000476593800006
WOS关键词	SYMPATRIC CARNIVORES ; MOLECULAR ANALYSIS ; MTDNA ; AUTHENTICATION ; CANIDAE ; BARCODE ; FECES
WOS类目	Ecology ; Evolutionary Biology
WOS研究方向	Environmental Sciences & Ecology ; Evolutionary Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215270
作者单位	1.Univ Catolica Temuco, Dept Ciencias Biol & Quim, Temuco, Chile; 2.Univ Catolica Temuco, Dept Ciencias Ambientales, Temuco, Chile; 3.Univ Catolica Temuco, Nucleo Estudios Ambientales, Temuco, Chile; 4.Univ Catolica Temuco, Dept Ciencias Agr & Acuicolas, Temuco, Chile; 5.Univ Catolica Temuco, Fac Ciencias Salud, Temuco, Chile; 6.Corp Nacl Forestal, Temuco, Chile; 7.Univ Catolica Temuco, Nucleo Prod Alimentaria, Temuco, Chile
推荐引用方式 GB/T 7714	Anabalon, Leonardo,Encina-Montoya, Francisco,Sanchez, Pamela,et al. High-resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chile[J],2019,9(13):7448-7454.
APA	Anabalon, Leonardo.,Encina-Montoya, Francisco.,Sanchez, Pamela.,Solano, Jaime.,Benavente, Felipe.,...&Vega, Rolando.(2019).High-resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chile.ECOLOGY AND EVOLUTION,9(13),7448-7454.
MLA	Anabalon, Leonardo,et al."High-resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chile".ECOLOGY AND EVOLUTION 9.13(2019):7448-7454.