干旱区生态环境知识资源中心(Arid): Complex immune responses and molecular reactions to pathogens and disease in a desert reptile (Gopherus agassizii)

Arid

DOI	10.1002/ece3.4897
	Complex immune responses and molecular reactions to pathogens and disease in a desert reptile (Gopherus agassizii)
	Drake, K. Kristina 1,2,3; Aiello, Christina M.1,4; Bowen, Lizabeth 5; Lewison, Rebecca L.2; Esque, Todd C.1; Nussear, Kenneth E.6; Waters, Shannon C.5; Hudson, Peter J.4
通讯作者	Drake, K. Kristina
来源期刊	ECOLOGY AND EVOLUTION
ISSN	2045-7758
出版年	2019
卷号	9 期号:5 页码:2516-2534
英文摘要	Immune function plays an important role in an animal's defense against infectious disease. In reptiles, immune responses may be complex and counterintuitive, and diagnostic tools used to identify infection, such as induced antibody responses are limited. Recent studies using gene transcription profiling in tortoises have proven useful in identifying immune responses to various intrinsic and extrinsic stressors. As part of a larger experiment with Mojave desert tortoises (Gopherus agassizii), we facilitated the transmission of the pathogenic bacteria, Mycoplasma agassizii (Myag), to naive adults and measured innate and induced immune reactions over time. Specifically, we evaluated clinical condition, presence of Myag in the nasal/oral cavity, induced antibody responses specific to Myag, and measured molecular reactions (gene transcript profiles) in 15 captive tortoises classified as naive, exposed, or infected and 14 wild tortoises for comparison. Myag was confirmed inside the nasal/oral cavity in exposed tortoises within 30-60 days of introduction to infected animals, yet we did not detect Myag specific induced antibody responses in these individuals until 420-595 days post exposure. Surprisingly, we found no overall differences in the gene transcript profiles between our experimental treatment groups throughout this study. This work highlights the complexities in assessing immune function and diagnosing pathogen related infections in tortoises and other reptiles.
英文关键词	desert tortoise immunity mRNA Mycoplasma agassizii transcription
类型	Article
语种	英语
国家	USA
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000460668800014
WOS关键词	RESPIRATORY-TRACT DISEASE ; SOFT-SHELLED TURTLE ; ARYL-HYDROCARBON RECEPTOR ; MYCOPLASMA-AGASSIZII ; ECOLOGICAL IMMUNOLOGY ; GENE-TRANSCRIPTION ; TORTOISE ; SYSTEM ; EXPOSURE ; EXPRESSION
WOS类目	Ecology ; Evolutionary Biology
WOS研究方向	Environmental Sciences & Ecology ; Evolutionary Biology
来源机构	United States Geological Survey ; University of California, Davis
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215250
作者单位	1.US Geol Survey, Western Ecol Res Ctr, Henderson, NV 89074 USA; 2.San Diego State Univ, Dept Biol, San Diego, CA 92182 USA; 3.Univ Calif Davis, Grad Grp Ecol, Davis, CA 95616 USA; 4.Penn State Univ, Dept Biol, University Pk, PA 16802 USA; 5.US Geol Survey, Western Ecol Res Ctr, Davis, CA USA; 6.Univ Nevada, Dept Geog, Reno, NV 89557 USA
推荐引用方式 GB/T 7714	Drake, K. Kristina,Aiello, Christina M.,Bowen, Lizabeth,et al. Complex immune responses and molecular reactions to pathogens and disease in a desert reptile (Gopherus agassizii)[J]. United States Geological Survey, University of California, Davis,2019,9(5):2516-2534.
APA	Drake, K. Kristina.,Aiello, Christina M..,Bowen, Lizabeth.,Lewison, Rebecca L..,Esque, Todd C..,...&Hudson, Peter J..(2019).Complex immune responses and molecular reactions to pathogens and disease in a desert reptile (Gopherus agassizii).ECOLOGY AND EVOLUTION,9(5),2516-2534.
MLA	Drake, K. Kristina,et al."Complex immune responses and molecular reactions to pathogens and disease in a desert reptile (Gopherus agassizii)".ECOLOGY AND EVOLUTION 9.5(2019):2516-2534.