干旱区生态环境知识资源中心(Arid): The Polyextreme Ecosystem, Salar de Huasco at the Chilean Altiplano of the Atacama Desert Houses Diverse Streptomyces spp. with Promising Pharmaceutical Potentials

Arid

DOI	10.3390/d11050069
	The Polyextreme Ecosystem, Salar de Huasco at the Chilean Altiplano of the Atacama Desert Houses Diverse Streptomyces spp. with Promising Pharmaceutical Potentials
	Cortes-Albayay, Carlos 1,2,3,4; Silber, Johanna 5; Imhoff, Johannes F.5; Asenjo, Juan A.3; Andrews, Barbara 3; Nouioui, Imen 4; Dorador, Cristina 1,2
通讯作者	Nouioui, Imen ; Dorador, Cristina
来源期刊	DIVERSITY-BASEL
EISSN	1424-2818
出版年	2019
卷号	11 期号:5
英文摘要	Salar de Huasco at the Chilean Altiplano of the Atacama Desert is considered a polyextreme environment, where solar radiation, salinity and aridity are extremely high and occur simultaneously. In this study, a total of 76 bacterial isolates were discovered from soil samples collected at two different sites in the east shoreline of Salar de Huasco, including H0 (base camp next to freshwater stream in the north part) and H6 (saline soils in the south part). All isolated bacteria were preliminarily identified using some of their phenotypic and genotypic data into the genera Streptomyces (86%), Nocardiopsis (9%), Micromonospora (3%), Bacillus (1%), and Pseudomonas (1%). Streptomyces was found dominantly in both sites (H0 = 19 isolates and H6 = 46 isolates), while the other genera were found only in site H0 (11 isolates). Based on the genotypic and phylogenetic analyses using the 16S rRNA gene sequences of all Streptomyces isolates, 18% (12 isolates) revealed <98.7% identity of the gene sequences compared to those in the publicly available databases and were determined as highly possibly novel species. Further studies suggested that many Streptomyces isolates possess the nonribosomal peptide synthetases-coding gene, and some of which could inhibit growth of at least two test microbes (i.e., Gram-positive and Gram-negative bacteria and fungi) and showed also the cytotoxicity against hepatocellular carcinoma and or mouse fibroblast cell lines. The antimicrobial activity and cytotoxicity of these Streptomyces isolates were highly dependent upon the nutrients used for their cultivation. Moreover, the HPLC-UV-MS profiles of metabolites produced by the selected Streptomyces isolates unveiled apparent differences when compared to the public database of existing natural products. With our findings, the polyextreme environments like Salar de Huasco are promising sources for exploring novel and valuable bacteria with pharmaceutical potentials.
英文关键词	bioactive compounds actinobacteria Atacama bioprospecting Chilean Altiplano
类型	Article
语种	英语
国家	Chile ; England ; Germany
开放获取类型	Green Submitted, gold, Green Published, Green Accepted
收录类别	SCI-E
WOS记录号	WOS:000472650300003
WOS关键词	SP NOV. ; STRAIN ; SEQUENCES ; POLYKETIDE ; BACTERIAL ; PEPTIDE ; SOIL
WOS类目	Biodiversity Conservation ; Ecology
WOS研究方向	Biodiversity & Conservation ; Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215059
作者单位	1.Univ Antofagasta, Lab Microbial Complex & Funct Ecol, Ctr Biotechnol & Bioengn CeBiB, Ave Angamos, Antofagasta 601, Chile; 2.Univ Antofagasta, Dept Biotechnol, Fac Marine Sci & Biol Resources, Ave Angamos, Antofagasta 601, Chile; 3.Univ Chile, Ctr Biotechnol & Bioengn CeBiB, Dept Chem Engn & Biotechnol, Santiago 851, Chile; 4.Newcastle Univ, Sch Nat & Environm Sci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England; 5.GEOMAR Helmholtz Zentrum Ozeanforsch Kiel, Dusternbrooker Weg 20, D-24105 Kiel, Germany
推荐引用方式 GB/T 7714	Cortes-Albayay, Carlos,Silber, Johanna,Imhoff, Johannes F.,et al. The Polyextreme Ecosystem, Salar de Huasco at the Chilean Altiplano of the Atacama Desert Houses Diverse Streptomyces spp. with Promising Pharmaceutical Potentials[J],2019,11(5).
APA	Cortes-Albayay, Carlos.,Silber, Johanna.,Imhoff, Johannes F..,Asenjo, Juan A..,Andrews, Barbara.,...&Dorador, Cristina.(2019).The Polyextreme Ecosystem, Salar de Huasco at the Chilean Altiplano of the Atacama Desert Houses Diverse Streptomyces spp. with Promising Pharmaceutical Potentials.DIVERSITY-BASEL,11(5).
MLA	Cortes-Albayay, Carlos,et al."The Polyextreme Ecosystem, Salar de Huasco at the Chilean Altiplano of the Atacama Desert Houses Diverse Streptomyces spp. with Promising Pharmaceutical Potentials".DIVERSITY-BASEL 11.5(2019).