干旱区生态环境知识资源中心(Arid): Determination of Flunixin in Swine Plasma, Urine and Feces by UPLC-MS/MS and its Application in the Real Samples

Arid

DOI	10.2174/1573412913666170918163625
	Determination of Flunixin in Swine Plasma, Urine and Feces by UPLC-MS/MS and its Application in the Real Samples
	Yu, Zugong; Luo, Xiaoqing; Guo, Fanxi; Zhang, Zhenrui; Peng, Lin
通讯作者	Yu, Zugong
来源期刊	CURRENT PHARMACEUTICAL ANALYSIS
ISSN	1573-4129
EISSN	1875-676X
出版年	2019
卷号	15 期号:1 页码:51-60
英文摘要	Background: Flunixin is a Non-Steroidal Anti-Inflammatory Drug (NSAID), because it can effectively alleviate the organism of pyrexia, inflammation and pain, it has been widely used in veterinary practice. In order to better study flunixin in the body's absorbed, distributed, metabolized and excreted, our team developed a UPLC-MS/MS method for determination of flunixin in swine plasma, urine and feces. Methods: Flunixin was extracted from the sample by liquid-liquid extraction and cleaned-up using a mixed-mode Oasis MCX solid-phase extraction column. Analysis was performed on UPLC-MS/MS operating in Multiple Reaction Monitoring (MRM) mode. Internal standard was used for quantitation of target drug. Results: Recoveries of fortified samples ranged from 90.2% to 101.4%, with Relative Standard Deviations (RSD) lower than 17.0%. The Limits Of Detection cLODs) and Quantification (LOQs) in plasma were 0.25 and 0.5 mu g L-1, in urine were 0.25 and 0.5 mu gL(-1), and in feces were 0.5 and 1 mu g kg(-1), respectively. This validated method was successfully applied to the determination of flunixin in real samples. The half-life of flunixin after the last dose in pigs was 7.37 +/- 1.60 h after intramuscular administration of 2.2 mg/kg of flunixin, and approximately 6.8% and 1.9% of the administered dose was excreted as parent compound in urine and feces respectively. Conclusion: The developed UPLC-MS/MS method for determination of flunixin in swine plasma, urine and feces was validated and successfully applied to monitor flunixin from real samples.
英文关键词	Flunixin swine plasma swine urine swine feces ultra-performance liquid chromatography-tandem mass spectrometry metabolism
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000458302600009
WOS关键词	LIQUID-CHROMATOGRAPHY ; ANTIINFLAMMATORY DRUGS ; EQUINE PLASMA ; HORSE URINE ; MEGLUMINE ; PHARMACOKINETICS ; EXTRACTION ; RESIDUES ; PHENYLBUTAZONE ; IDENTIFICATION
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215011
作者单位	Nanjing Agr Univ, Coll Vet Med, Lab Vet Pharmacol & Toxicol, Nanjing 210095, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Yu, Zugong,Luo, Xiaoqing,Guo, Fanxi,et al. Determination of Flunixin in Swine Plasma, Urine and Feces by UPLC-MS/MS and its Application in the Real Samples[J],2019,15(1):51-60.
APA	Yu, Zugong,Luo, Xiaoqing,Guo, Fanxi,Zhang, Zhenrui,&Peng, Lin.(2019).Determination of Flunixin in Swine Plasma, Urine and Feces by UPLC-MS/MS and its Application in the Real Samples.CURRENT PHARMACEUTICAL ANALYSIS,15(1),51-60.
MLA	Yu, Zugong,et al."Determination of Flunixin in Swine Plasma, Urine and Feces by UPLC-MS/MS and its Application in the Real Samples".CURRENT PHARMACEUTICAL ANALYSIS 15.1(2019):51-60.