干旱区生态环境知识资源中心(Arid): Molecular Cytogenetic Characterization of the Native Forage Grass Trichloris crinita

Arid

DOI	10.2135/cropsci2018.12.0731
	Molecular Cytogenetic Characterization of the Native Forage Grass Trichloris crinita
	Carolina Kozub, Perla 1; Laura Las Penas, Maria 2; Elda Novo, Patricia 3; Federico Cavagnaro, Pablo 4,5,6
通讯作者	Federico Cavagnaro, Pablo
来源期刊	CROP SCIENCE
ISSN	0011-183X
EISSN	1435-0653
出版年	2019
卷号	59 期号:4 页码:1604-1616
英文摘要	Trichloris crinita (Lag.) Parodi is a perennial forage grass native to-and of wide distribution in-arid and semiarid regions of the American continent. In addition to its good forage quality, this species is particularly valued in these drylands due to its high tolerance to drought, salinity, trampling, and grazing. Genetic and cytogenetic information for T. crinita is very scarce, hindering progress in genetic research and breeding of the species. We describe chromosome numbers, karyotypes, heterochromatin distribution, physical mapping of ribosomal DNA (rDNA) genes (18-5.8-26S and 5S) by fluorescent in situ hybridization (FISH), and chromosome pairing in meiotic cells for the first time in T. crinita accessions. Karyotype based on chromosome morphology and cytogenetic landmarks are presented for eight T. crinita accessions. Physical mapping of rDNA loci revealed variation in the number, size, and intensity of the 18-5.8-26S FISH signals, whereas 5S rDNA produced two signals per accession. Considering a basic chromosome number of 10, as reported for most Chloridoideae species and the facts that all 20 T. crinita accessions analyzed had 40 somatic chromosomes, that only bivalent chromosome pairings were observed in meiosis, and that chromosome morphology and sizes revealed high similarity between pairs of chromosomes, along with other reproductive features of the species, strongly suggest that T. crinita is an allotetraploid (2n = 4x = 40). Our cytogenetic data represent a useful resource for advancing cytogenetic research and breeding in T. crinita.
类型	Article
语种	英语
国家	Argentina
收录类别	SCI-E
WOS记录号	WOS:000473171700021
WOS关键词	IN-SITU HYBRIDIZATION ; 45S RDNA SITES ; RIBOSOMAL DNA ; SUBFAMILY CHLORIDOIDEAE ; CYNODON-DACTYLON ; 5S RDNA ; POACEAE ; HETEROCHROMATIN ; POLYPLOIDY ; LOCALIZATION
WOS类目	Agronomy
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214997
作者单位	1.Univ Nacl Cuyo UNCuyo, CONICET, Consejo Nacl Invest Cient & Tecn, IBAM,FCA, Mendoza, Argentina; 2.UNC, Consejo Nacl Invest Cient & Tecn, CONICET, Inst Multidisciplinario Biol Vegetal IMBIV, Cordoba, Argentina; 3.Univ Nacl Nordeste UNNE, CONICET, Consejo Nacl Invest Cient & Tecn, Inst Bot Nordeste IBONE,FCA, Corrientes, Argentina; 4.EEA La Consulta, INTA, La Consulta, Mendoza, Argentina; 5.Consejo Nacl Invest Cient & Tecn, Consejo Nacl Invest Cient & Tecn, Buenos Aires, DF, Argentina; 6.Univ Nacl Cuyo, Inst Hort, Fac Ciencias Agr, Mendoza, Argentina
推荐引用方式 GB/T 7714	Carolina Kozub, Perla,Laura Las Penas, Maria,Elda Novo, Patricia,et al. Molecular Cytogenetic Characterization of the Native Forage Grass Trichloris crinita[J],2019,59(4):1604-1616.
APA	Carolina Kozub, Perla,Laura Las Penas, Maria,Elda Novo, Patricia,&Federico Cavagnaro, Pablo.(2019).Molecular Cytogenetic Characterization of the Native Forage Grass Trichloris crinita.CROP SCIENCE,59(4),1604-1616.
MLA	Carolina Kozub, Perla,et al."Molecular Cytogenetic Characterization of the Native Forage Grass Trichloris crinita".CROP SCIENCE 59.4(2019):1604-1616.