干旱区生态环境知识资源中心(Arid): Investigating the cytotoxic effects of the venom proteome of two species of the Viperidae family (Cerastes cerastes and Cryptelytrops purpureomaculatus) from various habitats

Arid

DOI	10.1016/j.cbpc.2019.02.013
	Investigating the cytotoxic effects of the venom proteome of two species of the Viperidae family (Cerastes cerastes and Cryptelytrops purpureomaculatus) from various habitats
	Ozverel, Cenk Serhan 1,5; Damm, Maik 2; Hempel, Benjamin-Florian 2; Gocmen, Bayram 3; Sroka, Robert 2; Suessmuth, Roderich D.2; Nalbantsoy, Ayse 4
通讯作者	Hempel, Benjamin-Florian ; Nalbantsoy, Ayse
来源期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
ISSN	1532-0456
EISSN	1878-1659
出版年	2019
卷号	220 页码:20-30
英文摘要	Animal secretions are of great interest in terms of drug development due to their complex protein and peptide composition. Especially, in the field of therapeutic medications such as anti-cancer drugs snake venoms receive attention. In this study, we address two Viperidae species from various habitats with a particular focus on the cytotoxic potential along with the decomplexation of the venom proteome: the horned desert viper (Cerastes cerastes), native to desert regions of North Africa and the mangrove pit viper (Cryptelytrops purpureomaculatus), found in coastal forests of Southeast Asia. Initial cytotoxic screenings of the crude venoms revealed diverse activity, with the highest effect against SHSY5Y human glioblastoma carcinoma cells compared to other cancerous and non-cancerous cell lines. In-depth cytotoxicity studies of SHSY5Y cells with purified venom fractions revealed heterodimeric disintegrins from C. cerastes venom, which exerted a high cytotoxic activity with IC50 values from 0.11 to 0.58 mu M and a disintegrin-like effect on SHSY5Y morphology was observed due to cell detachment. Furthermore, two polyproline BPP-related peptides, one PLA(2) and a peptide-rich fraction were determined for C. purpureomaculatus with moderate IC50 values between 3 and 51 mu M. Additionally, the decryption of the venom proteomes by snake venomic mass spectrometry and comparison of the same species from different habitats revealed slight differences in the composition.
英文关键词	Cerastes cerastes Cryptelytrops purpureomaculatus Cytotoxicity Disintegrins Polyproline Snake venomics Venom
类型	Article
语种	英语
国家	Turkey ; Germany ; Cyprus
开放获取类型	Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000469306000003
WOS关键词	AMINO-ACID OXIDASE ; SNAKE-VENOM ; TOXINS ; TRIMERESURUS ; INHIBITION ; EXPRESSION ; ANTIVENOM ; AMMODYTES ; TOXICITY ; TAXONOMY
WOS类目	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214950
作者单位	1.Ege Univ, Fac Sci, Dept Biol, TR-35100 Izmir, Turkey; 2.Tech Univ Berlin, Inst Chem, Str 17 Juni 124, D-10623 Berlin, Germany; 3.Ege Univ, Fac Sci, Dept Biol, Zool Sect, TR-35100 Izmir, Turkey; 4.Ege Univ, Fac Engn, Dept Bioengn, TR-35100 Izmir, Turkey; 5.Near East Univ, Res Ctr Expt Hlth Sci, Near East Blvd, CY-99138 Nicosia, Cyprus
推荐引用方式 GB/T 7714	Ozverel, Cenk Serhan,Damm, Maik,Hempel, Benjamin-Florian,et al. Investigating the cytotoxic effects of the venom proteome of two species of the Viperidae family (Cerastes cerastes and Cryptelytrops purpureomaculatus) from various habitats[J],2019,220:20-30.
APA	Ozverel, Cenk Serhan.,Damm, Maik.,Hempel, Benjamin-Florian.,Gocmen, Bayram.,Sroka, Robert.,...&Nalbantsoy, Ayse.(2019).Investigating the cytotoxic effects of the venom proteome of two species of the Viperidae family (Cerastes cerastes and Cryptelytrops purpureomaculatus) from various habitats.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,220,20-30.
MLA	Ozverel, Cenk Serhan,et al."Investigating the cytotoxic effects of the venom proteome of two species of the Viperidae family (Cerastes cerastes and Cryptelytrops purpureomaculatus) from various habitats".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 220(2019):20-30.