干旱区生态环境知识资源中心(Arid): Validation of a liquid chromatography-tandem mass spectrometry method for analyzing cannabinoids in oral fluid

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DOI	10.1016/j.cca.2019.01.002
	Validation of a liquid chromatography-tandem mass spectrometry method for analyzing cannabinoids in oral fluid
	Sobolesky, Philip M.1; Smith, Breland E.1,2; Hubbard, Jacqueline A.1; Stone, Judy 1; Marcotte, Thomas D.3; Grelotti, David J.3; Grant, Igor 3; Fitzgerald, Robert L.1
通讯作者	Sobolesky, Philip M.
来源期刊	CLINICA CHIMICA ACTA
ISSN	0009-8981
EISSN	1873-3492
出版年	2019
卷号	491 页码:30-38
英文摘要	A liquid chromatography tandem mass spectrometry method was developed for quantifying ten cannabinoids in oral fluid (OF). This method utilizes OF collected by the Quantisar (TM) device and concurrently quantifies cannabinol (CBN), cannabidiol (CBD), Delta(9)-tetrahydrocannabinol (TIIC), 11-hydroxy-Delta(9)-THC (11-OH-THC), 11-nor-9-carboxy-Delta(9)-THC (THC-COOH), 11-nor-9-carboxy-Delta(9)-THC glucuronide (THC-COOH-gluc), Delta(9)-THC glucuronide (THC-gluc), cannabigerol (CBG), tetrahydrocannabiverin (THCV), and Delta(9)-tetrahydrocannabinolic acid A (THCA-A). Solid phase extraction was optimized using Oasis Prime HLB 30 mg 96-well plates. Cannabinoids were separated by liquid chromatography over a BEH C18 column and detected by a Waters TQ-S micro tandem mass spectrometer. The lower limits of quantification (LLOQ) were 0.4 ng/mL for CBN, CBD, THC, 11-OH-THC, THC-gluc, and THCV; and 1.0 ng/mL for THC-COOH, THC-COOH-gluc, CBG and THCA-A. Linear ranges extended to 2000 ng/mL for THC and 200 ng/mL for all other analytes. Inter-day analytical bias and imprecision at three levels of quality control (QC) was within +/- 15%. Mean extraction efficiencies ranged from 26.0-98.8%. Applicability of this method was tested using samples collected from individuals randomly assigned to smoke either a joint containing < 0.1%, 5.9%, or 13.4% THC content. This method was able to identify and calculate the concentration of 6 of 10 cannabinoids validated in this method.
英文关键词	Cannabinoid THC Oral fluid Tandem mass spectrometry
类型	Article
语种	英语
国家	USA
开放获取类型	Green Published
收录类别	SCI-E ; SSCI
WOS记录号	WOS:000460714900006
WOS关键词	ROAD TRAFFIC CRASH ; 11-NOR-DELTA(9)-TETRAHYDROCANNABINOL-9-CARBOXYLIC ACID ; WHOLE-BLOOD ; DISPOSITION ; RISK ; PLASMA
WOS类目	Medical Laboratory Technology
WOS研究方向	Medical Laboratory Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214927
作者单位	1.Univ Calif San Diego, Dept Pathol, Ctr Adv Lab Med, San Diego Hlth, San Diego, CA 92103 USA; 2.Insource Diagnost, Monrovia, CA USA; 3.Univ Calif San Diego, Dept Psychiat, Ctr Med Cannabis Res, San Diego, CA 92103 USA
推荐引用方式 GB/T 7714	Sobolesky, Philip M.,Smith, Breland E.,Hubbard, Jacqueline A.,et al. Validation of a liquid chromatography-tandem mass spectrometry method for analyzing cannabinoids in oral fluid[J],2019,491:30-38.
APA	Sobolesky, Philip M..,Smith, Breland E..,Hubbard, Jacqueline A..,Stone, Judy.,Marcotte, Thomas D..,...&Fitzgerald, Robert L..(2019).Validation of a liquid chromatography-tandem mass spectrometry method for analyzing cannabinoids in oral fluid.CLINICA CHIMICA ACTA,491,30-38.
MLA	Sobolesky, Philip M.,et al."Validation of a liquid chromatography-tandem mass spectrometry method for analyzing cannabinoids in oral fluid".CLINICA CHIMICA ACTA 491(2019):30-38.