干旱区生态环境知识资源中心(Arid): Sensitive Determination of Four Polypeptide Antibiotic Residues in Milk Powder by High Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry

Arid

DOI	10.1007/s10337-019-03777-y
	Sensitive Determination of Four Polypeptide Antibiotic Residues in Milk Powder by High Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry
	Liu, Tong 1; Zhang, Chuanbin 1,2; Zhang, Feng 1; Nie, Bo 3; Yuan, Fei 1; Huang, Hailan 2; Li, Hongna 1
通讯作者	Zhang, Feng
来源期刊	CHROMATOGRAPHIA
ISSN	0009-5893
EISSN	1612-1112
出版年	2019
卷号	82 期号:10 页码:1479-1487
英文摘要	Polypeptide antibiotics abuse can lead to antibiotic residues in food products and have unwanted effects on human health. A selective, accurate, and sensitive analytical method for the simultaneous determination of four polypeptide antibiotics (gramicidin S, bacitracin, polymyxin B, and polymyxin E) in infant formula powder was developed using solid-phase extraction (SPE) combined with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The samples were extracted with acidified methanol, deproteinized with acetonitrile, and degreased with n-hexane before SPE. After cleanup with 0.1% formic acid and methanol (3:1, v/v) in Oasis HLB cartridges, the extracts were analyzed by HPLC-MS/MS with electrospray ionization (ESI) source and time-scheduled multiple reaction monitoring (MRM). Linearity was assessed by using matrix-matched standard calibration and good determination coefficients (r(2) > 0.995) were obtained. The average recoveries for blank sample at three spiked concentration levels were in the range of 82.8-101.2%. The limits of detection (LODs) and limits of quantitation (LOQs) of all analytes were in the range of 5-15 mu g kg(-1) and 20-50 mu g kg(-1), respectively. The intra-day and inter-day precisions were lower than 10%. The results of method validation demonstrated that the developed method is accurate and reliable, and it can be applied for screening and quantitation of target polypeptide antibiotics in food. Graphic
英文关键词	Polypeptide antibiotics HPLC-MS MS Milk powder Gramicidin S
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000485313700005
WOS关键词	POLYMYXIN-B ; CAPILLARY-ELECTROPHORESIS ; QUANTITATIVE-ANALYSIS ; COLISTIN SULFATE ; ZINC BACITRACIN ; DRUG RESIDUES ; TISSUES ; TOXICITY ; NEOMYCIN ; SAMPLES
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214890
作者单位	1.Chinese Acad Inspect & Quarantine, Inst Food Safety, Beijing 100176, Peoples R China; 2.Qingdao Univ, Sch Chem & Chem Engn, Qingdao 266071, Shandong, Peoples R China; 3.Beijing Univ Chinese Med, Dongzhimen Hosp, Key Lab Chinese Internal Med, Minist Educ & Beijing, Beijing 100700, Peoples R China
推荐引用方式 GB/T 7714	Liu, Tong,Zhang, Chuanbin,Zhang, Feng,et al. Sensitive Determination of Four Polypeptide Antibiotic Residues in Milk Powder by High Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry[J],2019,82(10):1479-1487.
APA	Liu, Tong.,Zhang, Chuanbin.,Zhang, Feng.,Nie, Bo.,Yuan, Fei.,...&Li, Hongna.(2019).Sensitive Determination of Four Polypeptide Antibiotic Residues in Milk Powder by High Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry.CHROMATOGRAPHIA,82(10),1479-1487.
MLA	Liu, Tong,et al."Sensitive Determination of Four Polypeptide Antibiotic Residues in Milk Powder by High Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry".CHROMATOGRAPHIA 82.10(2019):1479-1487.