干旱区生态环境知识资源中心(Arid): Distribution of neuropeptide F in the ventral nerve cord and its possible role on testicular development and germ cell proliferation in the giant freshwater prawn, Macrobrachium rosenbergii

Arid

DOI	10.1007/s00441-019-02999-8
	Distribution of neuropeptide F in the ventral nerve cord and its possible role on testicular development and germ cell proliferation in the giant freshwater prawn, Macrobrachium rosenbergii
	Thongrod, Sirorat 1; Wanichanon, Chaitip 1; Sobhon, Prasert 1,2
通讯作者	Sobhon, Prasert
来源期刊	CELL AND TISSUE RESEARCH
ISSN	0302-766X
EISSN	1432-0878
出版年	2019
卷号	376 期号:3 页码:471-484
英文摘要	Neuropeptide F in invertebrates is a homolog of neuropeptide Y in mammals and it is a member of FMRFamide-related peptides. In arthropods, such as insects, there are two types of neuropeptide F comprising long neuropeptide F (NPF) and short neuropeptide F (sNPF). Both NPFs are known to play a crucial role in the regulations of foraging, feeding-related behaviors, circadian rhythm, stress responses, aggression and reproduction in invertebrates. We have earlier found that in the giant freshwater prawn, Macrobrachium rosenbergii, there are three isoforms of NPF and four isoforms of sNPF and that NPFs are expressed in the eyestalks and brain. In the present study, we investigate further the tissue distribution of NPF-I in the ventral nerve cord (VNC) and its role in the development of testes in small male (SM) Macrobrachium rosenbergii. By immunolocalization, using the rabbit polyclonal antibody against NPF-I as a probe, we could detect NPF-I immunoreactivity in the neuropils and neuronal clusters of the subesophageal ganglia (SEG), thoracic ganglia (TG) and abdominal ganglia (AG) of the SM prawns. In functional assays, the administrations of synthetic NPF-I (KPDPTQLAAMADALKYLQELDKYYSQVSRPRFamide) and sNPF (APALRLRFamide) peptides significantly increased the growth rates of SM prawns and significantly increased the gonadosomatic index (GSI) and proliferations of early germ cells in the seminiferous tubules of their testes. It is, therefore, suggestive that NPFs may play critical roles in energy homeostasis towards promoting growth as well as testicular development in prawns that could be applied in the aquaculture of this species.
英文关键词	Neuropeptide F Ventral nerve cord Subesophageal ganglion Testis Macrobrachium rosenbergii
类型	Article
语种	英语
国家	Thailand
收录类别	SCI-E
WOS记录号	WOS:000468934500014
WOS关键词	GONADOTROPIN-RELEASING HORMONES ; LOBSTER HOMARUS-AMERICANUS ; CHEMORECEPTOR CELLS ; TISSUE DISTRIBUTION ; ABDOMINAL-GANGLION ; GASTROPOD MOLLUSK ; DESERT LOCUST ; HELIX-ASPERSA ; CRAYFISH ; IDENTIFICATION
WOS类目	Cell Biology
WOS研究方向	Cell Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214853
作者单位	1.Mahidol Univ, Fac Sci, Dept Anat, Bangkok 10400, Thailand; 2.Burapha Univ, Fac Allied Hlth Sci, Long Hard Rd, Saen Suk 20131, Chonburi, Thailand
推荐引用方式 GB/T 7714	Thongrod, Sirorat,Wanichanon, Chaitip,Sobhon, Prasert. Distribution of neuropeptide F in the ventral nerve cord and its possible role on testicular development and germ cell proliferation in the giant freshwater prawn, Macrobrachium rosenbergii[J],2019,376(3):471-484.
APA	Thongrod, Sirorat,Wanichanon, Chaitip,&Sobhon, Prasert.(2019).Distribution of neuropeptide F in the ventral nerve cord and its possible role on testicular development and germ cell proliferation in the giant freshwater prawn, Macrobrachium rosenbergii.CELL AND TISSUE RESEARCH,376(3),471-484.
MLA	Thongrod, Sirorat,et al."Distribution of neuropeptide F in the ventral nerve cord and its possible role on testicular development and germ cell proliferation in the giant freshwater prawn, Macrobrachium rosenbergii".CELL AND TISSUE RESEARCH 376.3(2019):471-484.