干旱区生态环境知识资源中心(Arid): Tools to understand seasonality in health: quantification of microbe loads and analyses of compositional ecoimmunological data reveal complex patterns in Mojave Desert Tortoise (Gopherus agassizii) populations

Arid

DOI	10.1139/cjz-2018-0255
	Tools to understand seasonality in health: quantification of microbe loads and analyses of compositional ecoimmunological data reveal complex patterns in Mojave Desert Tortoise (Gopherus agassizii) populations
	Sandmeier, F. C.1; Leonard, K. L.1; Tracy, C. R.2; Drake, K. K.3; Esque, T. E.3; Nussear, K.4; Germano, J. M.5
通讯作者	Sandmeier, F. C.
来源期刊	CANADIAN JOURNAL OF ZOOLOGY
ISSN	0008-4301
EISSN	1480-3283
出版年	2019
卷号	97 期号:9 页码:841-848
英文摘要	Using data from six wild Mojave Desert Tortoise (Gopherus agassizii (Cooper, 1861)) populations, we quantified seasonal differences in immune system measurements and microbial load in the respiratory tract, pertinent to this species' susceptibility to upper respiratory tract disease. We quantified bacteria-killing activity of blood plasma and differential leukocyte counts to detect trends in temporal variation in immune function. We used centered log-ratio (clr) transformations of leukocyte counts and stress that such transformations are necessary for compositional data. We tested animals for the potential pathogen Pasteurella testudinis Snipes and Biberstein, 1982 with a newly created quantitative polymerase chain reaction (qPCR) assay, as well as for the known respiratory pathogens Mycoplasma agassizii Brown et al., 2001 and Mycoplasma testudineum Brown et al., 2004. We found very little disease and suggest that P. testudinis is a prevalent, commensal microbe in these Mojave Desert Tortoise populations, and its quantification may be a tool to study natural fluctuations in microbe levels in Mojave Desert Tortoise respiratory tracts. Our analyses showed that both the potential for inflammatory responses and microbe levels are highest in the spring for healthy Mojave Desert Tortoises, when lymphocyte levels are lowest. The genetic and statistical tools that we used are easily applicable to other wildlife systems and provide the necessary data to quantify species-wide trends in health and test hypotheses pertinent to host-microbe dynamics.
英文关键词	Gopherus agassizii Mojave Desert Tortoise ecoimmunology ectotherm temporal variation
类型	Article
语种	英语
国家	USA ; New Zealand
收录类别	SCI-E
WOS记录号	WOS:000487682100011
WOS关键词	PRINCIPAL COMPONENT ANALYSIS ; RESPIRATORY-TRACT DISEASE ; FREE-RANGING DESERT ; PASTEURELLA-TESTUDINIS ; MYCOPLASMA-AGASSIZII ; B-CELLS ; SP-NOV ; VERTEBRATES ; BACTERIA ; IMMUNITY
WOS类目	Zoology
WOS研究方向	Zoology
来源机构	United States Geological Survey ; Colorado State University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214777
作者单位	1.Colorado State Univ Pueblo, Biol Dept, 2200 Bonforte Blvd, Pueblo, CO 81001 USA; 2.Univ Nevada, Biol Dept, 1664 North Virginia St, Reno, NV 89557 USA; 3.US Geol Survey, 160 North Stephanie St, Henderson, NV 89074 USA; 4.Univ Nevada, Geog Dept, 1664 North Virginia St, Reno, NV 89557 USA; 5.New Zealand Dept Conservat, 18 Manners St, Wellington 6011, New Zealand
推荐引用方式 GB/T 7714	Sandmeier, F. C.,Leonard, K. L.,Tracy, C. R.,et al. Tools to understand seasonality in health: quantification of microbe loads and analyses of compositional ecoimmunological data reveal complex patterns in Mojave Desert Tortoise (Gopherus agassizii) populations[J]. United States Geological Survey, Colorado State University,2019,97(9):841-848.
APA	Sandmeier, F. C..,Leonard, K. L..,Tracy, C. R..,Drake, K. K..,Esque, T. E..,...&Germano, J. M..(2019).Tools to understand seasonality in health: quantification of microbe loads and analyses of compositional ecoimmunological data reveal complex patterns in Mojave Desert Tortoise (Gopherus agassizii) populations.CANADIAN JOURNAL OF ZOOLOGY,97(9),841-848.
MLA	Sandmeier, F. C.,et al."Tools to understand seasonality in health: quantification of microbe loads and analyses of compositional ecoimmunological data reveal complex patterns in Mojave Desert Tortoise (Gopherus agassizii) populations".CANADIAN JOURNAL OF ZOOLOGY 97.9(2019):841-848.