干旱区生态环境知识资源中心(Arid): Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia

Arid

DOI	10.1186/s40529-019-0257-y
	Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia
	Chen, Yen-Ming 1; Huang, Jian-Zhi 2,3; Hou, Ting-Wen 1; Pan, I-Chun 1
通讯作者	Pan, I-Chun
来源期刊	BOTANICAL STUDIES
EISSN	1999-3110
出版年	2019
卷号	60
英文摘要	BackgroundHaworthia are desert succulents belonging to the Asphodelaceae family. Haworthia species are cultivated commercially as ornamentals and some rare species are quite valuable at retail market but growth slowly and difficult to propagation. However, an efficient micropropagation protocol was remained insufficient.ResultsThe organogenic cultures obtained from inflorescence explants were cultured on Murashige and Skoog (MS) medium supplemented with various combinations of 6-benzylaminopurine (BA) and alpha-naphthalene acetic acid (NAA) under a light intensity of 10 mu molm(-2)s(-1) or 45 mu molm(-2)s(-1). The highest callus proliferation index (93.15%) with 1.0mgL(-1) BA+0.1mgL(-1) NAA under a light intensity of 10 mu molm(-2)s(-1). The best shoot proliferation rates were on media with either 1mgL(-1) BA+0-0.4mgL(-1) NAA (65.57-81.01%) under a light intensity of 45 mu molm(-2)s(-1). The highest root length (15.57mm) and the highest rooting frequency (17 roots per shoot) were obtained when adventitious shoots were inoculated on MS medium with 0.4mgL(-1) NAA+0.4mgL(-1) IBA. The survival rate of the transplanted plantlets was about 100%. The efficient micropropagation protocol proliferated Haworthia regenerate plants from inflorescence within 11weeks.ConclusionsThe present study determined the best combination of light intensity and plant growth regulators (PGRs) for improved organogenesis of Haworthia during propagation by tissue culture. This optimized protocol showed light intensity is an important factor for efficient callus or shoot regeneration. These results indicate that it will be useful to optimize the light conditions for future commercial cultivation, germplasm conservation, genetic engineering and molecular biology research of this ornamental plant.
英文关键词	Haworthia Inflorescence explants 6-Benzylaminopurine alpha-Naphthalene acetic acid Callus Shoot Light intensity
类型	Article
语种	英语
国家	Taiwan
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000475543000001
WOS关键词	IN-VITRO PROPAGATION ; SOMATIC EMBRYOGENESIS ; TISSUE-CULTURE ; INDUCTION ; MICROPROPAGATION ; ESTABLISHMENT ; ARABIDOPSIS ; AUXINS ; ALOE ; MECHANISMS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214727
作者单位	1.Natl Chung Hsing Univ, Dept Hort, 145 Xingda Rd, Taichung 402, Taiwan; 2.Natl Pingtung Univ Sci & Technol, Dept Plant Ind, 1 Shuefu Rd, Pingtung 912, Taiwan; 3.Natl Taiwan Univ, Coll Bioresources & Agr, Dept Agr Chem, 1,Sec 4,Roosevelt Rd, Taipei 106, Taiwan
推荐引用方式 GB/T 7714	Chen, Yen-Ming,Huang, Jian-Zhi,Hou, Ting-Wen,et al. Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia[J],2019,60.
APA	Chen, Yen-Ming,Huang, Jian-Zhi,Hou, Ting-Wen,&Pan, I-Chun.(2019).Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia.BOTANICAL STUDIES,60.
MLA	Chen, Yen-Ming,et al."Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia".BOTANICAL STUDIES 60(2019).