干旱区生态环境知识资源中心(Arid): Genome-wide association study (GWAS) of leaf cuticular wax components in Camelina sativa identifies genetic loci related to intracellular wax transport

Arid

DOI	10.1186/s12870-019-1776-0
	Genome-wide association study (GWAS) of leaf cuticular wax components in Camelina sativa identifies genetic loci related to intracellular wax transport
	Luo, Zinan 1; Tomasi, Pernell 1; Fahlgren, Noah 2; Abdel-Haleem, Hussein 1
通讯作者	Luo, Zinan ; Abdel-Haleem, Hussein
来源期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
出版年	2019
卷号	19
英文摘要	BackgroundIt is important to explore renewable alternatives (e.g. biofuels) that can produce energy sources to help reduce reliance on fossil oils, and reduce greenhouse gases and waste solids resulted from fossil oils consumption. Camelina sativa is an oilseed crop which has received increasing attention due to its short life cycle, broader adaptation regions, high oil content, high level of omega-3 unsaturated fatty acids, and low-input requirements in agriculture practices. To expand its Camelina production areas into arid regions, there is a need to breed for new drought-tolerant cultivars. Leaf cuticular wax is known to facilitate plant development and growth under water-limited conditions. Dissecting the genetic loci underlying leaf cuticular waxes is important to breed for cultivars with improved drought tolerance.ResultsHere we combined phenotypic data and single nucleotide polymorphism (SNP) data from a spring C. sativa diversity panel using genotyping-by-sequencing (GBS) technology, to perform a large-scale genome-wide association study (GWAS) on leaf wax compositions. A total of 42 SNP markers were significantly associated with 15 leaf wax traits including major wax components such as total primary alcohols, total alkanes, and total wax esters as well as their constituents. The vast majority of significant SNPs were associated with long-chain carbon monomers (carbon chain length longer than C-28), indicating the important effects of long-chain carbon monomers on leaf total wax biosynthesis. These SNP markers are located on genes directly or indirectly related to wax biosynthesis such as maintaining endoplasmic reticulum (ER) morphology and enabling normal wax secretion from ER to plasma membrane or Golgi network-mediated transport.ConclusionsThese loci could potentially serve as candidates for the genetic control involved in intracellular wax transport that might directly or indirectly facilitate leaf wax accumulation in C. sativa and can be used in future marker-assisted selection (MAS) to breed for the cultivars with high wax content to improve drought tolerance.
英文关键词	Leaf cuticular wax Intracellular wax transport Genome-wide association studies (GWAS) Camelina sativa
类型	Article
语种	英语
国家	USA
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000467423000001
WOS关键词	LINKAGE DISEQUILIBRIUM ; DROUGHT TOLERANCE ; POPULATION-STRUCTURE ; AGRONOMIC TRAITS ; COMPLEX TRAITS ; L. CRANTZ ; ACCUMULATION ; DIVERSITY ; BIOSYNTHESIS ; EXPRESSION
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214705
作者单位	1.USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA; 2.Danforth Plant Sci Ctr, St Louis, MO 63132 USA
推荐引用方式 GB/T 7714	Luo, Zinan,Tomasi, Pernell,Fahlgren, Noah,et al. Genome-wide association study (GWAS) of leaf cuticular wax components in Camelina sativa identifies genetic loci related to intracellular wax transport[J],2019,19.
APA	Luo, Zinan,Tomasi, Pernell,Fahlgren, Noah,&Abdel-Haleem, Hussein.(2019).Genome-wide association study (GWAS) of leaf cuticular wax components in Camelina sativa identifies genetic loci related to intracellular wax transport.BMC PLANT BIOLOGY,19.
MLA	Luo, Zinan,et al."Genome-wide association study (GWAS) of leaf cuticular wax components in Camelina sativa identifies genetic loci related to intracellular wax transport".BMC PLANT BIOLOGY 19(2019).