干旱区生态环境知识资源中心(Arid): Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance

Arid

DOI	10.1186/s12870-019-1900-1
	Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance
	Jia, Huixia 1; Zhang, Jin 1,2; Li, Jianbo 3; Sun, Pei 1; Zhang, Yahong 1; Xin, Xuebing 3; Lu, Mengzhu 1; Hu, Jianjun 1
通讯作者	Zhang, Jin ; Hu, Jianjun
来源期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
出版年	2019
卷号	19 期号:1
英文摘要	Background Drought is a major environmental constraint to plant growth, development and productivity. Compared with most willows that are generally susceptible to drought, the desert willow Salix psammophila has extraordinary adaptation to drought stress. However, its molecular basis of drought tolerance is still largely unknown. Results During polyethylene glycol 6000 (PEG 6000)-simulated drought stress, we found that the osmotic adjustment substances were accumulated and the antioxidant enzyme activities were enhanced in S. psammophila roots. A total of 8172 differentially expressed genes were identified in roots of S. psammophila through RNA-Sequencing. Based on K-means clustering, their expression patterns were classified into nine clusters, which were enriched in several stress-related processes including transcriptional regulation, response to various stresses, cell death, etc. Moreover, 672 transcription factors from 45 gene families were differentially expressed under drought stress. Furthermore, a weighted gene co-expression network was constructed, and eight genes were identified as hub genes. We demonstrated the function of two hub genes, magnesium-dependent phosphatase 1 (SpMDP1) and SpWRKY33, through overexpression in Arabidopsis thaliana. Overexpression of the two hub genes enhanced the drought tolerance in transgenic plants, suggesting that the identification of candidate drought tolerance genes in this study was highly efficient and credible. Conclusions Our study analyzed the physiological and molecular responses to drought stress in S. psammophila, and these results contribute to dissect the mechanism of drought tolerance of S. psammophila and facilitate identification of critical genes involved in drought tolerance for willow breeding.
英文关键词	Salix psammophila Drought Co-expression network Hub gene Transgene SpMDP1 SpWRKY33
类型	Article
语种	英语
国家	Peoples R China ; USA
开放获取类型	gold, Green Submitted, Green Published
收录类别	SCI-E
WOS记录号	WOS:000481846800002
WOS关键词	HIGH-SALINITY STRESSES ; HYDROTHERMAL LIQUEFACTION ; EXPRESSION ANALYSIS ; ARABIDOPSIS WRKY33 ; PROTEIN GENE ; WATER STRESS ; E3 LIGASE ; RICE ; POPLAR ; OVEREXPRESSION
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214702
作者单位	1.Chinese Acad Forestry, Natl Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat, Res Inst Forestry,State Key Lab Tree Genet & Bree, Beijing 100091, Peoples R China; 2.Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA; 3.Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China
推荐引用方式 GB/T 7714	Jia, Huixia,Zhang, Jin,Li, Jianbo,et al. Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance[J],2019,19(1).
APA	Jia, Huixia.,Zhang, Jin.,Li, Jianbo.,Sun, Pei.,Zhang, Yahong.,...&Hu, Jianjun.(2019).Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance.BMC PLANT BIOLOGY,19(1).
MLA	Jia, Huixia,et al."Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance".BMC PLANT BIOLOGY 19.1(2019).