干旱区生态环境知识资源中心(Arid): Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii

Arid

DOI	10.1186/s12864-019-6042-1
	Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii
	Bensaoud, Chaima 1,2; Aounallah, Hajer 1; Sciani, Juliana Mozer 3,4; Faria, Fernanda 3; Chudzinski-Tavassi, Ana Marisa 3; Bouattour, Ali 1; M’ghirbi, Youmna 1
通讯作者	Bensaoud, Chaima
来源期刊	BMC GENOMICS
ISSN	1471-2164
出版年	2019
卷号	20 期号:1
英文摘要	BackgroundThe hard tick Hyalomma dromedarii is one of the most injurious ectoparasites affecting camels and apparently best adapted to deserts. As long-term blood feeders, ticks are threatened by host defense system compounds that can cause them to be rejected and, ultimately, to die. However, their saliva contains a cocktail of bioactive molecules that enables them to succeed in taking their blood meal. A recent sialotranscriptomic study uncovered the complexity of the salivary composition of the tick H. dromedarii and provided a database for a proteomic analysis. We carried out a proteomic-informed by transcriptomic (PIT) to identify proteins in salivary glands of both genders of this tick species.ResultsWe reported the array of 1111 proteins identified in the salivary glands of H. dromedarii ticks. Only 24% of the proteins were shared by both genders, and concur with the previously described sialotranscriptome complexity. The comparative analysis of the salivary glands of both genders did not reveal any great differences in the number or class of proteins expressed their enzymatic composition or functional classification. Indeed, few proteins in the entire proteome matched those predicted from the transcriptome while others corresponded to other proteins of other tick species.ConclusionThis investigation represents the first proteomic study of H. dromedarii salivary glands. Our results shed light on the differences between the composition of H. dromedarii male and female salivary glands, thus enabling us to better understand the gender-specific strategy to feed successfully.
英文关键词	Hyalomma dromedarii Salivary glands Proteome LC-MS MS PIT
类型	Article
语种	英语
国家	Tunisia ; Czech Republic ; Brazil
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000485480500002
WOS关键词	ACARI IXODIDAE VECTORS ; RHIPICEPHALUS-SANGUINEUS ; IXODES-PERSULCATUS ; SOFT TICK ; PROTEIN ; INSIGHT ; ALPHA-2-MACROGLOBULIN ; IDENTIFICATION ; CLONING ; METALLOPROTEASES
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214692
作者单位	1.Univ Tunis El Manar, Inst Pasteur Tunis, Serv Entomol Med, LR11IPT03, Tunis 1002, Tunisia; 2.Czech Acad Sci, Biol Ctr, Inst Parasitol, Ceske Budejovice 37005, Czech Republic; 3.Inst Butantan, Lab Biol Mol, Av Vital Brazil 1500, BR-05503900 Sao Paulo, Brazil; 4.Univ Sao Francisco, Lab Multidisciplinar Pesquisa, Av Sao Francisco de Assis 218, BR-12916900 Paulista, SP, Brazil
推荐引用方式 GB/T 7714	Bensaoud, Chaima,Aounallah, Hajer,Sciani, Juliana Mozer,et al. Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii[J],2019,20(1).
APA	Bensaoud, Chaima.,Aounallah, Hajer.,Sciani, Juliana Mozer.,Faria, Fernanda.,Chudzinski-Tavassi, Ana Marisa.,...&M’ghirbi, Youmna.(2019).Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii.BMC GENOMICS,20(1).
MLA	Bensaoud, Chaima,et al."Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii".BMC GENOMICS 20.1(2019).