干旱区生态环境知识资源中心(Arid): Validation and delineation of a locus conferring Fusarium crown rot resistance on 1HL in barley by analysing transcriptomes from multiple pairs of near isogenic lines

Arid

DOI	10.1186/s12864-019-6011-8
	Validation and delineation of a locus conferring Fusarium crown rot resistance on 1HL in barley by analysing transcriptomes from multiple pairs of near isogenic lines
	Gao, Shang 1,2,3; Zheng, Zhi 1; Powell, Jonathan 1; Habib, Ahsan 1,2,3,4; Stiller, Jiri 1; Zhou, Meixue 2,3; Liu, Chunji 1
通讯作者	Liu, Chunji
来源期刊	BMC GENOMICS
ISSN	1471-2164
出版年	2019
卷号	20 期号:1
英文摘要	Background Fusarium crown rot (FCR) is a chronic and severe disease in cereal production in semi-arid regions worldwide. A putative quantitative trait locus conferring FCR resistance, Qcrs.cpi-1H, had previously been mapped on the long arm of chromosome 1H in barley. Results In this study, five pairs of near-isogenic lines (NILs) targeting the 1HL locus were developed. Analysing the NILs found that the resistant allele at Qcrs.cpi-1H significantly reduced FCR severity. Transcriptomic analysis was then conducted against three of the NIL pairs, which placed the Qcrs.cpi-1H locus in an interval spanning about 11 Mbp. A total of 56 expressed genes bearing single nucleotide polymorphisms (SNPs) were detected in this interval. Five of them contain non-synonymous SNPs. These results would facilitate detailed mapping as well as cloning gene(s) underlying the resistance locus. Conclusion NILs developed in this study and the transcriptomic sequences obtained from them did not only allow the validation of the resistance locus Qcrs.cpi-1H and the identification of candidate genes underlying its resistance, they also allowed the delineation of the resistance locus and the development of SNPs markers which formed a solid base for detailed mapping as well as cloning gene(s) underlying the locus.
英文关键词	Fusarium crown rot QTL validation Near-isogenic line RNA-seq Transcriptome Barley
类型	Article
语种	英语
国家	Australia ; Bangladesh
开放获取类型	Green Accepted, Green Published, Green Submitted, gold
收录类别	SCI-E
WOS记录号	WOS:000481410600005
WOS关键词	HEAD BLIGHT RESISTANCE ; LOOP NTPASE OSYCHF1 ; LARGE-EFFECT QTL ; BREAD WHEAT ; MAJOR QTL ; RNA-SEQ ; UDP-GLUCOSYLTRANSFERASE ; DEFENSE RESPONSES ; LONG ARM ; PATHOGEN
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
来源机构	Commonwealth Scientific and Industrial Research Organisation
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214691
作者单位	1.CSIRO Agr & Food, St Lucia, Qld 4067, Australia; 2.Univ Tasmania, Sch Land & Food, Hobart, Tas, Australia; 3.Univ Tasmania, Tasmanian Inst Agr, Hobart, Tas, Australia; 4.Khulna Univ, Biotechnol & Genet Engn Discipline, Khulna 9208, Bangladesh
推荐引用方式 GB/T 7714	Gao, Shang,Zheng, Zhi,Powell, Jonathan,et al. Validation and delineation of a locus conferring Fusarium crown rot resistance on 1HL in barley by analysing transcriptomes from multiple pairs of near isogenic lines[J]. Commonwealth Scientific and Industrial Research Organisation,2019,20(1).
APA	Gao, Shang.,Zheng, Zhi.,Powell, Jonathan.,Habib, Ahsan.,Stiller, Jiri.,...&Liu, Chunji.(2019).Validation and delineation of a locus conferring Fusarium crown rot resistance on 1HL in barley by analysing transcriptomes from multiple pairs of near isogenic lines.BMC GENOMICS,20(1).
MLA	Gao, Shang,et al."Validation and delineation of a locus conferring Fusarium crown rot resistance on 1HL in barley by analysing transcriptomes from multiple pairs of near isogenic lines".BMC GENOMICS 20.1(2019).