干旱区生态环境知识资源中心(Arid): Caragana korshinskii phenylalanine ammonialyase is up-regulated in the phenylpropanoid biosynthesis pathway in response to drought stress

Arid

DOI	10.1080/13102818.2019.1623718
	Caragana korshinskii phenylalanine ammonialyase is up-regulated in the phenylpropanoid biosynthesis pathway in response to drought stress
	Liu, Furong 1; Xie, Lifang 1; Yao, Zhenye 1; Zhou, Yulu 1,2; Zhou, Wenfei 1; Wang, Junhui 1; Sun, Yingying 1; Gong, Chunmei 1,3
通讯作者	Gong, Chunmei
来源期刊	BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
ISSN	1310-2818
EISSN	1314-3530
出版年	2019
卷号	33 期号:1 页码:842-854
英文摘要	Drought is one of the most severe abiotic stresses, the damage due to which, various plant species mitigate by activating mechanisms that are not yet well understood. Caragana korshinskii is a xerophytic shrub found in the semi-arid regions of northwest China with high tolerance to several abiotic stresses, including drought. Based on the de novo transcriptome data from C. korshinskii leaflets collected along a precipitation gradient on the Loess Plateau (China), most of the differentially expressed genes were explored using trend analysis along the precipitation gradient. Gene ontological analysis showed that phenylpropanoid biosynthesis process -> secondary metabolite biosynthetic process terms were the most significant gene ontologies, whereas Kyoto Encyclopedia of Genes and Genomes-based analysis indicated that the biosynthesis of secondary metabolites was a significant metabolic pathway. Real-time polymerase chain reaction and enzyme activity analyses confirmed the increased transcription of the phenylalanine ammonialyase (PAL) gene in C. korshinskii under drought stress in field and laboratory conditions. These results suggested that C. korshinskii adjusts its secondary metabolism to water-deficit environments and activates PAL by drought stress. Therefore, further studies on the obtained data can expand the current understanding of the molecular and genetic mechanisms responsible for the drought endurance in C. korshinskii.
英文关键词	Caragana korshinskii drought stress transcriptome phenylalanine ammonialyase
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000472175900001
WOS关键词	LOESS PLATEAU ; RESISTANCE ; SALT ; ACCUMULATION ; METABOLISM ; EXPRESSION ; FLAVONOIDS ; ENZYMES ; BIOLOGY ; PLANTS
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/214676
作者单位	1.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China; 2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China; 3.Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Liu, Furong,Xie, Lifang,Yao, Zhenye,et al. Caragana korshinskii phenylalanine ammonialyase is up-regulated in the phenylpropanoid biosynthesis pathway in response to drought stress[J]. 西北农林科技大学,2019,33(1):842-854.
APA	Liu, Furong.,Xie, Lifang.,Yao, Zhenye.,Zhou, Yulu.,Zhou, Wenfei.,...&Gong, Chunmei.(2019).Caragana korshinskii phenylalanine ammonialyase is up-regulated in the phenylpropanoid biosynthesis pathway in response to drought stress.BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,33(1),842-854.
MLA	Liu, Furong,et al."Caragana korshinskii phenylalanine ammonialyase is up-regulated in the phenylpropanoid biosynthesis pathway in response to drought stress".BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT 33.1(2019):842-854.